Xray crystallography Lasse Mårtensson, IFM-Chemistry.

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Presentation transcript:

Xray crystallography Lasse Mårtensson, IFM-Chemistry

Ribosome

© 2006 Academic Press

© 2006 Academic Press

Diffraction pattern from a protein obtained by xray crystallography © 2006 Academic Press

Electrondensity map

© 2006 Academic Press

© 2006 Academic Press

© 2006 Academic Press

© 2006 Academic Press

© 2006 Academic Press

Requirements: Pure protein of high concentration mg/ml AND… Protein crystals How to obtain protein crystals??

© 2006 Academic Press

© 2006 Academic Press

© 2006 Academic Press

© 2006 Academic Press

© 2006 Academic Press

Crystallization robot

Terms used in Xray crystallography Crystal system Spacegroup Unit cell Assymmetric unit Miller indices hkl Braggs law Ewald sphere

© 2006 Academic Press

© 2006 Academic Press

© 2006 Academic Press

© 2006 Academic Press

© 2006 Academic Press

© 2006 Academic Press

© 2006 Academic Press

© 2006 Academic Press

© 2006 Academic Press

© 2006 Academic Press

© 2006 Academic Press

© 2006 Academic Press

© 2006 Academic Press

© 2006 Academic Press

© 2006 Academic Press

© 2006 Academic Press

© 2006 Academic Press

© 2006 Academic Press

Ewald sphere with a radius of 1/ All lattice points that touch the lattice obey Bragg´s law

© 2006 Academic Press

Refinement of the structure

Quality index R-factor:

Ramachandran plot