Bimonthly Meeting on Oct. 10, 2008 Metabolic Concentrations and Ratios of Brain Tissue Amarjeet Bhullar

We have compared metabolite ratios, calculated using different methods: Directly Generated by Functool [F1] Using Metabolites Concentration [F2] Gaussian Function Overlapped on metabolite peak with Baseline correction Gaussian Function Overlapped on metabolite peak with Baseline+Noise correction

Baseline Correction Baseline corrects separately each individual spectral point in spectrum By calculating and subtracting a Spline through each points for smoothing. If smoothing width is non zero, takes its average value, which is very small =zero In most of our spectrum baseline exists at zero

Baseline+Noise Correction Metaboliteppm rangeImage range Choline Creatine NAA Lipid and lactate Noise=Average value of after removing of four peaks

Chemical Information on Voxel # 16, 19, 20

Chemical Information on Voxel # 24, 25, 26

Chemical Information on Voxel # 27, 30, 31

Metabolite Ratios Cho/Cre

Conclusion : There is no significant difference between our calculation and Functool. Suggestions are welcome