Rockets in front of IOLR

Participation in SB – P1 (NIO) WP 0 – Project Managements WP 1 – Mechanisms for establishment of dormant stages WP 2 – Mechanisms for arousal from dormant stages WP 4 – Engineering/Genetic testing strategies WP 5 – Discovery of common and divergent pathways for dormant stages, dormancy and desiccation resistance WP 6 – Dissemination of results

Workpackage 1 (WP1): Mechanisms for establishment of dormant stages DeliverablesDeliverable Number Deliverable date Optimal conditions for inducing asexually and sexually reproducing rotifers and their resting eggs D4M9 cDNA libraries, EST sequencingD5M16 Pattern of global gene expression during formation of resting eggs by microarray analysis D6M27 Quantitative analyses of the expression of specific genes by QPCR D7M35 Proteins and metabolites identified to be associated with formation of resting eggs or contained within them D8M36

P1- NIO: Goals for M6 WP1: TasksDeliverable Number Asexual and sexually reproducing rotifer cultures D4M9 Construction of cDNA libraries (P5): a)Asexually reproducing rotifers b)Sexually reproducing rotifers c)Subtractive library ?? EST’s (P5): ~ 5,000 clones per library (~15,000 ESTs for rotifers) D5 – Partner 5M16 Goals for M6-18

Workpackage 2 (WP2): Mechanisms for arousal from dormant stages DeliverablesDeliverable Number Deliverable date Patterns of global gene expression during exit from dormancy of rotifer resting eggs by microarray analyses D11M35 Quantitative expression levels of specific genes during resumed development by QPCR D12M35 Proteins and metabolites associated with resumed metabolic activities during embryonic development in restings eggs and asexual rotifer resting eggs D13M30

WP2: TasksDeliverable Number Resumed development of rotifer resting eggs- time course of events D11M35 Goals for M12

Nadav’s presentation

Workpackage 1 (WP1): Mechanisms for establishment of dormant stages DeliverablesDeliverable Number Deliverable date Optimal conditions for inducing asexually and sexually reproducing rotifers and their resting eggs D4M9 cDNA libraries, EST sequencingD5M16 Pattern of global gene expression during formation of resting eggs by microarray analysis D6M27 Quantitative analyses of the expression of specific genes by QPCR D7M35 Proteins and metabolites identified to be associated with formation of resting eggs or contained within them D8M36

WP1: TasksDeliverable Number Milestone Collection of samples for global gene expression and QPCR analyses; amictic and mictic females from cultures at different stages of resting egg formation; resting eggs and amictic eggs D6, D7M5 Collection of samples for proteome and compound analyses; amictic and mictic females from cultures at different stages of resting egg formation; resting eggs and amictic eggs D8, D9M6 Goals for M12-18

Workpackage 2 (WP2): Mechanisms for arousal from dormant stages DeliverablesDeliverable Number Deliverable date Patterns of global gene expression during exit from dormancy of rotifer resting eggs by microarray analyses D11M35 Quantitative expression levels of specific genes during resumed development by QPCR D12M35 Proteins and metabolites associated with resumed metabolic activities during embryonic development in restings eggs and asexual rotifer resting eggs D13M30

WP2: TasksDeliverable Number Milestone Collection of samples for global gene expression and QPCR analyses; resting eggs at 3 progressive stages of hatching D11, D12M7 Collection of samples for proteome and compound analyses; resting eggs at 3 progressive stages of hatching D13, D14M8 Goals for M12-18

Workpackage 4 (WP4):Engineering/Genetic testing strategies DeliverablesDeliverable Number Deliverable date Differences in the transcriptome, proteome and metabolome analyses between asexual and sexual rotifer strains D20M35

WP4: TasksDeliverable Number Milestone Collection of samples for global gene expression and QPCR analyses of amictic females from a genetic line showing sexual reproduction and amictic females from a sexually reproducing culture D20M12 Collection of samples for proteome and compound analyses of amictic females from a genetic line showing sexual reproduction and amictic females from a sexually reproducing culture D20M12 Goals for M12-18

Workpackage 5 (WP5):Discovery of common and divergent pathways for dormant stages, dormancy and desiccation resistance DeliverablesDeliverable Number Deliverable date Construction of the databasesD24M30 Identification of species- specific and conserved genes, proteins and compounds D25M33 Development of in silco models for cell dormancy or desiccation tolerance D26M36 Production of a comprehensive reportD7M35

WP5: TasksDeliverable Number Compile information on gene and cDNA sequences of rotifers for data base D24M30 Goals for M12-18

Workpackage 6 (WP6):Dissemination of results DeliverablesDeliverable Number Deliverable date Participation in scientific meetingsD28M36 A workshop on dormancy: opportunities and challenges: preservation of cells and organisms D29M24* Publication of a book on dormancyD30M36* * To be discussed at the meeting

WP 6: TasksDeliverable Number Deliverable date Participation in scientific meetings and publication of manuscripts D28M36 Organization of the workshop- application for additional financial support D29M24* * To be discussed at the meeting Goals for M12-18