RNA08: Efforts at Non- Redundancy Laura Murray Richardson Laboratory, Duke University RNA Ontology Consortium May

RNA backbone is a headache to determine… –High number of degrees of freedom and limits on data both hamper structure determination –Resulting noise and the high dimensionality of the problem hamper data- driven analysis …but too biologically important to ignore –Intimately involved in ribozyme function, aptamer binding, protein interactions, etc. -- Other molecules see the entire surface presented to them, whether we can or not!

A Series of Tubes 1Q9A 1JJ2

RIBO SOME

Selection and Quality Filtering Resolution (3Å and better… mostly) Categories Discrimination between similar structures: –Resolution –R –Size/sterics/geometry –Occasional weird cases

Consensus? Centralized repository Consult to create a single utility dataset –Resource for all groups –Updatable –Resolve weird files

RNA08 RNA03RNA05RNA08 est. Files Residues ~17000

Acknowledgements Richardson LabRutgers-PragueGeorgia TechYale Jane RichardsonHelen BermanLoren WilliamsAnna-Marie Pyle David RichardsonBohdan SchneiderEli HershkovitzJohn Westbrook Gary KapralZdenek MoravekKevin Keating Bryan ArendallDavid Micallef Lizbeth VideauDaniela Hamova Ian Davis Funding NIHHHMINSFROC

Outline Introduction: RNA backbone structure and conformations –Challenges and approaches –Building consensus Structural motifs and the RNA Ontology Consortium Building a non-redundant dataset