Thanking you for the attention

Participation in SB – P1 (NIO) WP 0 – Project Managements WP 1 – Mechanisms for establishment of dormant stage WP 2 – Mechanisms for arousal from dormant stages WP 4 – Engineering/Genetic testing strategies WP 5 – Discovery of common and divergent pathways for dormant stages, dormancy and desiccation resistance WP 6 – Dissemination of results

Workpackage 1 (WP1): Mechanisms for establishment of dormant stage DeliverablesDeliverable Number Deliverable date Optimal conditions for inducing asexually and sexually reproducing rotifers and their resting eggs D4M9 cDNA libraries, EST sequencingD5M16 Pattern of global gene expression during formation of resting eggs by miroarray analysis D6M27 Quantitative analyses of the expression of specific genes by QPCR D7M35 Proteins and metabolites identified to be associated with formation of resting eggs or contained within them D8M36

Workpackage 2 (WP2): Mechanisms for arousal from dormant stages DeliverablesDeliverable Number Deliverable date Patterns of global gene expression during exit from dormancy of rotifer resting eggs by microarray analyses D11M35 Quantitative expression levels of specific genes during resumed development by QPCR D12M35 Proteins and metabolites associated with resumed metabolic activities during embryonic development in restings eggs and asexual rotifer resting eggs D13M30

Workpackage 4 (WP4):Engineering/Genetic testing strategies DeliverablesDeliverable Number Deliverable date Differences in the transcriptome, proteome and metabolome analyses between asexual and sexual rotifer strains D20M35

Workpackage 5 (WP5):Discovery of common and divergent pathways for dormant stages, dormancy and desiccation resistance DeliverablesDeliverable Number Deliverable date Construction of the databasesD24M30 Identification of species- specific and conserved genes, proteins and compounds D25M33 Development of in silco models for cell dormancy or desiccation tolerance D26M36 Production of a comprehensive reportD7M35

Workpackage 6 (WP6):Dissemination of results DeliverablesDeliverable Number Deliverable date Participation in scientific meetingsD28M36 A workshop on dormancy: opportunities and challenges: preservation of cells and organisms D29M24* Publication of a book on dormancyD30M36* * To be decided at the meeting

P1- NIO: Goals for M6 WP1: TasksDeliverable Number Asexual and sexually reproducing rotifer cultures D4M9 Construction of cDNA libraries (P5): a)Asexually reproducing rotifers b)Sexually reproducing rotifers c)Subtractive library ?? EST’s (P5): ~ 5,000 clones per library (~15,000 ESTs for rotifers) D5M16 Goals for M12-16

P1- NIO: Goals for M12-16 WP5: TasksDeliverable Number Compile information on gene and cDNA sequences of rotifers for data base D24M30 WP2: TasksDeliverable Number Collection of asexual rotifer eggs and resting eggs samples for proteome profiling D4M9