Tucson High School Biotechnology Course Spring 2010.

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Presentation transcript:

Tucson High School Biotechnology Course Spring 2010

What is gel electrophoresis? wells DNA (-)

Loading Buffer pcr products + STAIN

DNA Sequencing

DNA Sequencing: Basic Principle Sequence of base pairs?? AACGGATCATTCG polymerase

first human genome = ten $1 billion The quest for the $1K human genome 2nd: 10,000 instrument days (30 instruments for 1 $10 million J. Craig Venter 3rd: 454 pyrosequencing, two $2 million James Watson Nature, Nov “effectively” 4 human genomes each) - first African genome (Illumina, UK) - first Asian genome (Beijing Genomics, China) - first cancer genome (Washington University, USA) The $1,000 human genome, coming soon?

454 Pyrosequencing - the generations

The “pyro” in pyrosequencing

Images courtesy of Roche (Technical presentation)

Pyrosequencing - emPCR

Pyrosequencing - image processing

Sequence production (Billions of bases/month) Cost: Cents per base Courtesy of Phil Hugenholtz (JGI) Sequencing costs