Bio-Luminescence Detection Project Manager: Eitan Novotny Sean Dang Yu-Kun Zhang Philip Tran

Overview Biological rhythms –Samples emit periodic light –Periods around 24 hours –Sample rate once every 10 minutes Commercial device flaws –Missing flow through function –Product is costly Design goals –Integration with original equipment –Improve function and reduce cost MDR Specification Design Changes

Box Design 39cm x 39cm x 32cm Designed in Pro Engineering Two Pieces –Main box –Lid (top and front)

Box Design - Details Internal temperature must match incubator Two 80mm square holes plus one 80mm fan for airflow Light isolated environment 5 BNC connectors for PMTs and Motor 2 18cm x 4cm windows for tube baffles

Box Design - Overview Light baffle

Main Box

Lid

Motor 1240i board works with specific motors Parallel motor connection not possible Principal needs –Single high torque motor –Four rotating mirrors

Motor - Solution Motor is Oriental Motor PK243A1A-SG18 –Used in commercial application Step up gear used for high precision Central gear and outer gear are 5” Shafts connect outer gears to mirrors Contact switches used for error checking

Light Source/Detector Luciferin - emits light Wavelength range: 268nm to 537nm Photomultiplier Tube (PMT) Detects wavelength range from 185nm to 680nm Effective area 4mm x 20mm

Light Efficiency/Path Length Commercial application has a.25 cm path length Our light path length is about 3cm to 5cm Light goes to the mirror and reflects up to the PMT Loss of light due to vertical arrangement To minimize loss of light, add mirrors

Light Efficiency - Continued Interference from other light sources Add reflective walls, to minimize interference from other light sources

Electronics H8259 from Hamamatsu PCI-6601 Counter Board Applied Motion 1240i Oriental Stepper Motor Computer and software

Budget PartCost (each)QtyPrice H8259$11504$ i$2001$200 PCI-6601$6001$600 Motor$1771$177 Metal$46.116$277 Gears$62.025$310 Shaft Holder$27.465$137 Shaft$20.051$20 Wires$100$100 Total*$6421 *total cost to date

Conclusion MDR Specification done before 2 nd semester Accomplishments –Gearing design done –Box design done Mirror design will be completed before conclusion of semester Device should be completed on time Device should be under budget Aluminum for box construction