The Egg. Egg Microvilli Zona Pellucida Zp Zona Pellucida Glycoproteins.

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Presentation transcript:

The Egg

Egg Microvilli

Zona Pellucida Zp

Zona Pellucida Glycoproteins

The Sperm

Axonemes and Sliding Microtubules

Sperm Surface Asymmetry

Sea Urchins

Sea Urchin Acrosome Reaction

Sea Urchin Fertilization Direct Binding of Sperm to Egg

Competition Assay to Identify Egg Receptor

Isolated Zona Pellucida Blocks Sperm Binding + ZP (2 cell)

Isolated ZP3 Blocks Sperm Binding

Man NAcGlc O-linked Oligosaccharide NAcGlc Man NAcGlc O-linked Oligosaccharide NAcGlc Gal + 2UDP-Gal Galactosyl Transferase Galactosyl Transferase

Role of Galactosyl Transferase in Binding to Egg

Acrosome Reaction & Penetration of Zona Pellucida

Vesiculation of Acrosome

Fertilins and ADAMs family proteins

Fertilin-  hydrophobic domain

Model for Fertilin Function During Fertilization

Incorporation of Sperm Into Egg – Role of Microvilli

Incorporation of Sperm Components Into Egg

Formation of Sperm Pronucleus

Persistence of Sperm Mitochondria In Embryos

Propagation of Calcium “Wave”