Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings PowerPoint ® Lecture Slide Presentation prepared by Christine L. Case Microbiology B.E Pruitt & Jane J. Stein AN INTRODUCTION EIGHTH EDITION TORTORA FUNKE CASE Chapter 6, part B Microbial Growth
Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Deep-freezing: -50°to -95°C Lyophilization (freeze-drying): Frozen (-54° to -72°C) and dehydrated in a vacuum Preserving Bacteria Cultures
Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Binary fission Budding Conidiospores (actinomycetes) Fragmentation of filaments Reproduction in Prokaryotes
Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Binary Fission Figure 6.11
Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Figure 6.12b
Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings If 100 cells growing for 5 hours produced 1,720,320 cells:
Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Figure 6.13
Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Figure 6.14
Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Plate Counts: Perform serial dilutions of a sample Direct Measurements of Microbial Growth Figure 6.15, top portion
Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Inoculate Petri plates from serial dilutions Plate Count Figure 6.16
Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings After incubation, count colonies on plates that have colonies (CFUs) Plate Count Figure 6.15
Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Filtration Direct Measurements of Microbial Growth Figure 6.17a, b
Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Multiple tube MPN test Count positive tubes and compare to statistical MPN table. Direct Measurements of Microbial Growth Figure 6.18b
Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Direct Measurements of Microbial Growth Direct Microscopic Count
Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Direct Measurements of Microbial Growth Figure 6.19
Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Turbidity Estimating Bacterial Numbers by Indirect Methods Figure 620
Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Estimating Bacterial Numbers by Indirect methods Metabolic activity Dry weight