Relationship of Gene’s Type and Introns Chi K. To

Eukaryotes contain multiple introns per gene. Throughout eukaryotic evolution, the length of introns had been changed by either insertion, deletion or both. How big of these lengths had been change? Do they just happen in specific type of genes? What are those types?

Method: Method: Use the Browser to get introns in the whole genome. Do pairwise alignment between human and dog genomic sequences with the reconstructed sequences of their common ancestor. Only select introns have gaps that are greater than 100bp.

Insertion (gaps on ancestral sequences), deletion (gaps on human/dog sequences). Get genes’ IDs Upload data to the - help to calculate the Hypergeometric distribution for the data Gene ontology classification of introns.

Result: GO ID Descriptionp-val GO: Mol. Function: GTPase regulator activity [Modulates the rate of GTP hydrolysis by a GTPase] 1.89E-05 GO: Location: plasma membrane 1.01E-04 GO: Integral to plasma membrane 2.3E-04 GO: Biological process: NLS-bearing substrate-nucleus 5.42E-04 Insertion in Human

Go ID Descriptionp-val GO: Mol. Function: GTPase regulator activity 5.49E-06 GO: Mol. Function: small GTPase regulatory/interacting protein activity 3.65E-04 GO: Mol. Function: guanyl-cucleotide exchange factor activity 6.89E-04 GO: Mol. Function: cytoskeletal protein binding 1.96E-03 Deletion in Human

Go ID Descriptionp-val GO: Mol. Function: small GTPase regulatory/interacting protein activity 5.35E-04 GO: Biological process: NLS-bearing substrate-nucleus 5.35E-04 GO: Bio. Process: Actin cytoskeleton organization and biogenesis 2.67E-03 GO: Cellular component: collagen type IV 2.67E-03 Insertion in Dog

Go ID Descriptionp-val GO: Actin cytoskeleton organization and biogenesis 2.53E-04 GO: Bio. Process: Actin filament-based process 2.75E-04 GO: Mol. Function: small GTPase regulatory/interacting protein activity 4.06E-04 GO: Location: plasma membrane 4.8E-04 Deletion in Dog

* In human: Genes have the molecular function related to GTPase regulator activity or locate in plasma membrane tend to change the lengths of introns within the genes during the evolutionary process. * In human: Genes have the molecular function related to GTPase regulator activity or locate in plasma membrane tend to change the lengths of introns within the genes during the evolutionary process. * In Dog: Genes have the biological process related to Actin cytoskeleton organization and biogenesis and the mol. munction related to small GTPase regulatory/interacting protein activity tend to change the lengths of introns within the genes during the evolution.

A big deletion of a intron in gene NM_ of Dog

Special thanks to: Special thanks to: 1/ Baertsch, Robert – pairwise alignment data 1/ Baertsch, Robert – pairwise alignment data 2/ - for the G.O. statistics 2/ - for the G.O. statisticshttp://gostat.wehi.edu.au/ 3/ UCSC genome Browser 3/ UCSC genome Browser