Tel. 021/692 51 98 Some Applications: Transverse sections through the right thigh. Transverse sections through the right leg. Frontal section through.

Slides:

Advertisements

Similar presentations

Northern blotting. Electrophoresis We can separate DNA and RNA molecules by size using agarose gel electrophoresis.

Advertisements

Mass Spectrometry Kyle Chau and Andrew Gioe. Computation of Molecular Mass -Mass Spectrum is a plot of intensity as a function of mass- charge ratio,

Gel electrophoresis Ashti Mohammad Amin M.Sc. Molecular Biology Medical Research Center Hawler Medical University

1% Agarose Gel DNA Electrophoresis Running time : 52 minutes Distance measured from the well to the top of the second band: 4,5 cm Running voltage: 100.

William S. Klug Michael R. Cummings Charlotte A. Spencer Concepts of Genetics Eighth Edition Chapter 10 DNA Structure and Analysis Copyright © 2006 Pearson.

Gel Electrophoresis.

CISC667, F05, Lec24, Liao1 CISC 667 Intro to Bioinformatics (Fall 2005) DNA Microarray, 2d gel, MSMS, yeast 2-hybrid.

PROTEOMICS LECTURE. Genomics DNA (Gene) Functional Genomics TranscriptomicsRNA Proteomics PROTEIN Metabolomics METABOLITE Transcription Translation Enzymatic.

Definition of PCR Requirements for PCR PCR Process Agarose gel electrophoresis.

Basic Scheme of Biological Phenomena

2D-Gel Analysis Jennifer Wagner Image retrieved from

Western Blotting.

Electrophoresis Ayat Zawawi.

Comparing DNA Aim: How can we compare organisms using gel electrophoresis? Do Now: Write two observations from your results of gel electrophoresis.

Molecular Techniques in Microbiology These include 9 techniques (1) Standard polymerase chain reaction Kary Mullis invented the PCR in 1983 (USA)Kary.

Analysis of amino acids and proteins

AP Biology DNA Study Guide. Chapter 16 Molecular Basis of Heredity The structure of DNA The major steps to replication The difference between replication,

Protein Primary Sequence Protein analysis road map: Bioassay design Isolation/purification Analysis Sequencing.

Proteomics The science of proteomics Applications of proteomics Proteomic methods a. protein purification b. protein sequencing c. mass spectrometry.

Implementation of radiotracers use in methods for differential analysis of protein expression Mauro Fasano Centre of NeuroScience and DBSF University of.

Quantitative analysis of 2D gels Generalities. Applications Mutant / wild type Physiological conditions Tissue specific expression Disease / normal state.

---/-∙/-∙-∙/∙∙∙∙/∙-∙/---/--/---/∙∙∙/---/--/∙/∙∙∙/

Lecturer: David. * Reverse transcription PCR * Used to detect RNA levels * RNA is converted to cDNA by reverse transcriptase * Then it is amplified.

Changes in glutamine synthetase activity and in protein pattern of wheat leaves after Fusarium infection Attila Pécsváradi Zoltán Nagy, Edit Németh Dept.

A Novel Approach to Identifying Differential Gene Expression Robert J. Pignolo and Oriana Galardi-Este.

Healthy ControlCase 2D gel images of Serum protein sample Set of gel images for analysis.

AppliChem © There is another top address in Darmstadt!  Proteo-Dyes Background: 2-D gel of E.coli proteins stained with Proteo-Dye RuBPS.

Electrophoresis By John Darwin, Wes Gallagher, and Thompson Grant.

Gel Electrophoresis By Erin Martin & Satya Moolani.

Polyacrylamide Gel Electrophoresis. Electrophoresis Horizontal Agarose Gels Agarose forms a gel or molecular sieve that supports the movement of small.

DNA PROBE, LABELING, SIGNAL

Chromosomes, Genes, and Mutations

1 Expression Standard Ken Conover March Expression Standard Protein Purification SDS-PAGE Protocol His-Tag & Gel Code Blue Stains Making the Expression.

Phenotype Testing and SDS- PAGE Mod2 Day4 Thursday Oct 16th, 2008.

DNA Sequencing A population of DNA fragments is generated. –One end is common to all fragments (the 5’ end of the sequencing primer). –The other end terminates.

Date of download: 6/1/2016 Copyright © 2016 American Medical Association. All rights reserved. From: Aquaporin 4 Expression and Tissue Susceptibility to.

By: Alicia Gray. Map courtesy of the Chronic Wasting Disease Alliance.

Estimation of quantity and quality of isolated DNA

Plasmid Purification Miniprep

Information flow from DNA to trait

Lec 1 Molecular biology.

Invest. Ophthalmol. Vis. Sci ;45(6): doi: /iovs Figure Legend:

21.8 Recombinant DNA DNA can be used in

“Proteomics is a science that focuses on the study of proteins: their roles, their structures, their localization, their interactions, and other factors.”

REF and ATP5b status on mitochondrial fraction.

محاضرة عامة التقنيات الحيوية (هندسة الجينات .. مبادئ وتطبيقات)

DNA Sequencing A population of DNA fragments is generated.

DNA Sequencing A population of DNA fragments is generated.

Chapter 3 DNA, RNA and Protein

Technology that uses electricity to separate molecules in a gel slab

Set-1. Quiz-Bacterial protein gel analysis

Reading Gels -once a gel has been run, it is stained with another chemical exposed to UV light to allow the DNA to appear -below is an example of a gel.

A. C. B. D. Bifidobacterium mono-culture Bifidobacterium mono-culture

Analysis of differentially expressed proteins involved in hand, foot and mouth disease and normal sera L. Deng, H.-L. Jia, C.-W. Liu, K.-H. Hu, G.-Q.

Electrophoresis / SDS-PAGE

A Molecular World p Objectives:

Transgene Expression in the Brain Stem Effected by Intramuscular Injection of Polyethylenimine/DNA Complexes Shu Wang, Nan Ma, Shujun J. Gao, Hanry Yu,

Analysis of differentially expressed proteins involved in hand, foot and mouth disease and normal sera L. Deng, H.-L. Jia, C.-W. Liu, K.-H. Hu, G.-Q.

Rodríguez-Milla Miguel A. , Salinas Julio Molecular Plant

Molecular Biotechnology

Volume 3, Issue 5, Pages (September 2010)

Protein identification using MS/MS.

Proteomic analysis of externalized proteins

Proteomic analysis of invasive TCCs

Welcome to Bio 290, Introduction to Genetics!

Targeting strategy and molecular verification of myoglobin disruption.

Molecular Biotechnology 4C x MWM

Identification of a New Splice Form of the EDA1 Gene Permits Detection of Nearly All X- Linked Hypohidrotic Ectodermal Dysplasia Mutations Alex W. Monreal,

Presentation transcript:

Tel. 021/

Some Applications:

Transverse sections through the right thigh. Transverse sections through the right leg. Frontal section through the right foot

Separation of RNA and DNA. Detection by UV light.

IEF SDS-PAGE Identification of proteins and their modifications

Observe the difference in GFAP isoforms in Alzheimer ’ disease young old Alzheimer

Quantification Identification Comparison Molecular weights Scan import, TIFF

Example: Quantification of spots

This program is used in Geneva, and serves to identify differences in the protein composition of 2D gels