Evidence for the presence of XMRV in 33 PCR Negative US CFS Patients 19/33 Antibodies in the plasma 30/33 Transmissable virus in the plasma 10/33 Protein expression in Decitibine (5Aza2DC) treated PBMC Thus, since the submission to Science we determined 99 of 101 US patients show evidence of XMRV infection

Since the submission to Science, we have +-replicated at the NCI/CC our findings in other cohorts not in the WPI repository. 9/15 (60%) positive for XMRV gag DNA from fresh PBMC 13/15 (86.7%) positive by western for XMRV Env and Gag upon co-culture of plasma or PBMC with LNCaP 8/15 (53%) plasma samples contain antibody to XMRV Env XMRV Expression in NC/FL Cohort 9/15 (60%) positive for XMRV gag DNA from fresh PBMC 13/15 (86.7%) positive by western for XMRV Env and Gag upon co-culture of plasma or PBMC with LNCaP 8/15 (53%) plasma samples contain antibody to XMRV Env Since the submission to Science, we have replicated at the NCI/CC our findings in other cohorts not in the WPI repository.

Familial Transmission of XMRV From Plasma from patients with Childhood Alzheimer’s Acute flulike infection in the family Mother/Father XMRV Env/Antibody positive (no active virus detected) XMRV envelope protein in LNCaP from children’s plasma

XMRV Associated Neuroimmune Diseases (XAND): Potential Candidates: Atypical MS: 3/3 positive for XMRV ENV protein and gag DNA Fibromyalgia: 12/20 (60%) positive for XMRV gag DNA Autism: 6/15 (40%) positive for XMRV gag DNA – 4/7 ( 57%) positive for serum Antibody to XMRV Env Gulf War Illness : Not Tested Samples were taken from family members of XMRV positive CFS subjects with these neuroimmune diseases.

~ 77 of the Nevada cohort have a clonal TCRg rearrangement More than 100 of these patients have clonal populations of gamma delta T cells Gamma delta T cells play an active role in the regulation and resolution of pathogen induced immune responses. They accumulate at sites of inflammation associated with viral, bacterial and parasitic infections and in auto immune diseases Interestingly, Gamma Delta T cells up-regulate MIP1lalpha and Beta, TNF alpha, TCR  Clonality in Nevada CFS Cohort