Neurobiology 2007 Micha Spira 21 March Learning and Memory The Aplysia Model STF

Neurobiology 2007 Micha Spira 25 March PKC and STF Aplysia Model

Alterations at the post synaptic level

Micha Spira Neurobiology 2007 Long term 28 March 2007

Activation of nuclear transcription factor cAMP responsive element CRE –binding protein (CREB1) phosphorylated by PKA, MAPK or Cam kinase CREB1 function as transcriptional activator Leads to LTF Injection of CRE oligonucleotides bund to CREB1 and blok LTF but not STF Concomitantly CREB2 is down regulated by MAPK Injection of antiCREB2 antibodies is sufficient to trigger facilitation

Synaptic capture of learning induces gene products The activation of a specific synapse tag the synapse Covalent modification Local protein synthesis to stabilize the mark

Local protein synthesis at active synapse mRNA species are transported by special mechanisms to the synaptic sites. They are translated only after docking and in response to a specific signal. 5x5HT at one synapse activate local protein synthesis. If inhibited no LTF.

End of Aplysia Model