Fluorescence : One phenomenon, many applications Jussi Kinnunen, MSc (Physics), PhD student Oral presentation CREATE Gjøvik

There will be: Short introduction to fluorescence Few applications –Something about bioanalytics –Coloring or whitening merchandise –Arts Measurements and data analysis –by using two monochromator –method Fluorescence : One phenomenon, many applications / Jussi Kinnunen 2

What is fluorescence?

For me it is fancy colours Fluorescence : One phenomenon, many applications / Jussi Kinnunen 4

Light interaction with matter When light comes Absorption –Time about s Scattering –Time about 0 s When light goes Fluorescence emission –Lifetime about ( ) s –Lower energy photons than the one(s) absorbed Phosphorescence –Lifetime >10 -8 s –Lower energy photons than the one(s) absorbed Scattering –Energy if the photon is close to the incident one(s) Fluorescence : One phenomenon, many applications / Jussi Kinnunen 5

Applications: Bioanalytics

Fast detection of small quantities of anything Idea: –Choose a receptor for the target –Attach label (reporter) to the receptor –Illuminate and see Where is your target –e.g. cancer cells Was there any of your target –e.g. DNA sequencing Fluorescence : One phenomenon, many applications / Jussi Kinnunen 7

Applications: Industry

Making things white Fluorescence : One phenomenon, many applications / Jussi Kinnunen 9

Applications: Art

Masterpiece (Miera, 1950’s), white light and UV Photo courtesy of Nick Padalino Fluorescence : One phenomenon, many applications / Jussi Kinnunen 11

Color measurements of fluorescence

Two monochromator method Fluorescence : One phenomenon, many applications / Jussi Kinnunen 13

Measured data Donaldson matrix is calculated from the measured data The reflectance –On diagonal The fluorescence –Off diagonal Fluorescence : One phenomenon, many applications / Jussi Kinnunen 14

Simulations

Simulation with the scientific brown Fluorescence : One phenomenon, many applications / Jussi Kinnunen 16

Simulation with the scientific brown Fluorescence : One phenomenon, many applications / Jussi Kinnunen 17

Lights Simulations under –Tungsten light (green) –Daylight simulator (blue) –White led (red) –Fluorescent light (cyan) Fluorescence : One phenomenon, many applications / Jussi Kinnunen 18

Under “smooth” light sources Halogen lightDaylight simulator Fluorescence : One phenomenon, many applications / Jussi Kinnunen 19 reflectance fluorescence total The black curve is measured under polycromatic light

Under “peaky” light sources Led lightFluorescent light Fluorescence : One phenomenon, many applications / Jussi Kinnunen 20 reflectance fluorescence total The black curve is measured under polycromatic light

Thank you.