Replicação na presença de erros. Recombination at the Molecular Level Breakage and joining also directed by enzymes. Homologous recombination.

Slides:



Advertisements
Similar presentations
DNA Recombination Roles Types Homologous recombination in E.coli
Advertisements

Bacteria replication, recombination, and transformation
Molecular Evolution 2 Recombination & Transposition
MBV2010/BIO2140 Colloquium, March 6. RULES Multiple choice Only one correct answer seconds to answer No textbook, computer, mobile phone, please.
DNA/RNA Metabolism Blackburn & Gait, Ch. 5 Neidle, Ch. 4 Recombination understand basics of the reaction know advantages/disadvantages of recombination.
Retroviruses and Retroposons Chapter Introduction Figure 22.1.
Transposons & Mechanisms of Transposition
DNA Repair. Spontaneous Alterations Depurination and Deammination.
DNA Repair and Recombiantion. Methyl-directed mismatch repair (1) If any mismatch escapes the proof reading mechanisms it will cause distortion of the.
Microbial Genetics (Micr340)
Microbial Genetics (Micr340)
CS262 Discussion Section 3. Topics for today DNA replication DNA sequencing: Biological tools Transposons “Out of Africa” hypothesis of human origins.
Genetic Recombination 3 by: Nouf alyami. Content I. INTRODUCTION. II. GENERAL RECOMBINATION III. SITE-SPECIFIC RECOMBINATION.
DNA Replication. Why is DNA Replication needed? When cells are dividing… During Interphase of Mitosis & Meiosis DNA must be copied so that each new cell.
DNA Replication, Repair, and Recombination
Chapter 9 Genetics of Bacteria and Their Viruses Jones and Bartlett Publishers © 2005.
DNA Recombination Roles Types Homologous recombination in E.coli
Gene and Chromosome. DNA is the genetic material.
Genetic Variability in Bacteria Mutation spontaneous vs inducible point mutations silent, missense, nonsense, frameshift forward vs reverse (reversion.
Transposition Evidence Mechanisms: DNA-mediated RNA-mediated.
DNA & RNA Protein Synthesis. DNA be.com/watch?v =4PKjF7OumYo.
Biological Roles for Recombination 1.Generating new gene/allele combinations (crossing over during meiosis) 2.Generating new genes (e.g., Immuno- globulin.
DNA Recombination.
Translesion DNA Synthesis Cells bypass lesions encountered at the replication fork during DNA synthesis and correct them after replication is finished.
DNA Technology. 1.Isolation – of the DNA containing the required gene 2.Insertion – of the DNA into a vector 3.Transformation – Transfer of DNA into a.
Chapter 5 General Recombination.
CHAPTER 19 M ECHANISMS OF R ECOMBINATION. Recombination occurs at regions of homology between chromosomes through the breakage and reunion of DNA molecules.
BACTERIA AND VIRUSES. DNA core Protein coat (capsid) Characteristics: Parasitic Replicate only inside phenomenal rate.
Genetic recombination: 1.Homologous Recombination 2. Site-Specific Recombination 3. DNA Transposition.
Shatha Khalil Ismael. Transformation Certain species of Gram- negative, gram- positive bacteria and some species of Archaea are transformable. The uptake.
BACTERIAL TRANSPOSONS
2A. Distinguish between DNA and RNA.
Bacterial genetics and molecular biology. Terminology Genetics:Study of what genes are, how they carry information, how information is expressed, and.
Chapter 11 Site-Specific Recombination & Transposition of DNA
DNA metabolism DNA replication DNA repair DNA recombination.
Lecture 9 Site Specific Recombination and Transposition Quiz 5 due today at 4 PM.
Introduction to Molecular Genetics Studiju materiāli / MolekularasBiologijas / Ievads MolGen / EN.
DNA UNIT: Learning Outcomes It is expected that students will: name the four bases in DNA and describe the structure of DNA using the following terms:
Chapter 15 Transposons.
III. DNA Replication A. Each chain of nucleotides of the DNA double helix has all the information needed to reconstruct the complementary chain of nucleotides.
The Genetics of Bacteria and Their Viruses
Transposable Elements DNA Sequences That Change Positions in the Genome.
Repair of Damaged DNA DNA is the only cellular macromolecule that can be repaired DNA damage includes: base modifications nucleotide deletions or insertions.
Bacterial Transposons Author Meenakshi Agarwal, Mehta Gunjan Mentor Dr. Santanu Ghosh.
Maintenance of genomes Copying the genome sequence (replication) Repairing damage to the genome sequence Rearranging genome sequences.
©2001 Timothy G. Standish John 15:4 4Abide in me, and I in you. As the branch cannot bear fruit of itself, except it abide in the vine; no more can ye,
MBV2010 Colloquium, March 10. RULES Multiple choice Only one correct answer seconds to answer No textbook, computer, mobile phone, please There.
Homologous Recombination
Chapter 10 DNA Metabolism: Replication, Recombination, and Repair DNA polymerase & DNA Recombination.
Presentation Introduction of DNA Recombination Haoran Zhang Department of Chemical and Biological Engineering Tufts University.
When using adult genetic material to clone a mammal, which of the following is a problem? 1.clones tend to have health problems 2.not all genes in the.
Last Class DNA replication Chromosome replication DNA repair General Recombination.
Chapter 18.1 Contributors of Genetic Diversity in Bacteria.
MICROBIOLOGIA GENERALE
Office Hours (midterm #1)
TRANSPOSABLE GENETIC ELEMENTS I
TRANSFERIMIENTO LATERAL DE GENES
Transposable Elements
2A. Distinguish between DNA and RNA.
2A. Distinguish between DNA and RNA.
2A. Distinguish between DNA and RNA.
Evolution of eukaryote genomes
DNA Recombination -- in real life --
Homologous Recombination
Transposable Elements
Homologous Recombination
Investigation 1 Claims:
Figure: Title: Transposons have inverted repeats and generate target repeats Caption: Transposons have inverted terminal repeats and generate direct.
Presentation transcript:

Replicação na presença de erros

Recombination at the Molecular Level Breakage and joining also directed by enzymes. Homologous recombination occurs during synapsis in meiosis I, general recombination in bacteria, and viral genetic exchange. Molecular mechanism proposed by Holliday and Whitehouse (1964). Depends on complementary base pairing.

RecA protein-mediated DNA synapsis Rec A has multiple DNA binding sites, hence can hold a single strand and a double helix together Rec A is also a DNA-dependent ATPase

Recombinational DNA repair

Site-specific recombination

Integração ou excisão de um bacteriofago

Replicative transposition – the element moves a copy of itself to a new site via a DNA intermediate Conservative transposition – the element itself moves from the donor site into the target site

Retrotransposition – the element makes na RNA copy of itself which is reversed transcribed into a DNA which is then inserted

When IS elements get it together: composite transposons Two IS elements flank an antibiotic resistance gene Tn10 (E.coli)