Introducing a new instrument to the Cancer Center Flow Cytometry Core Facility: The LSR II by Becton Dickinson

BD LSR II New technology in the BD LSRII optics and digital electronics have created a more sensitive flow cytometer that yields more information from each sample. The new Windows ® based digital acquisition system provides increased detection and channel resolution by sampling signals at 10 million times a second.

Up to 8-color detection The LSRII is fully configurable with three fixed-aligned, air cooled lasers. The LSRII is fully configurable with three fixed-aligned, air cooled lasers. The 488nm blue laser detects four wavelengths at once, allowing for more versatile experimentation. The 488nm blue laser detects four wavelengths at once, allowing for more versatile experimentation. The optical system of the 633 HeNe red laser can detect both APC and APC-Cy7 simultaneously. The optical system of the 633 HeNe red laser can detect both APC and APC-Cy7 simultaneously. A third 405nm violet laser has two detectors, making it capable to analyze experiments including ECFP, Alexa Fluor 405, Pacific blue or side population.

8-color Analysis! Current configurations allow for up to 8-color analysis and in the future can be upgraded for detection of up to 18 colors!

Eight-color detection with many combinations of fluorochromes and fluorescent proteins.

BD FACSDiva ™ Flow Cytometry Acquisition & Analysis Software Simplify operational efficiency using: PC-like browser, Reusable acquisition and analysis templates, Automated compensation setup, And Snap-To gating.

Automatic Compensation Automated compensation uses unstained and single positive controls to determine proper compensation. Automated compensation uses unstained and single positive controls to determine proper compensation. Once set, the software adjusts the compensation as needed between experiments. Once set, the software adjusts the compensation as needed between experiments. Offline compensation When manually compensating a sample, a user need only take the data file and compensate later. When manually compensating a sample, a user need only take the data file and compensate later. This is efficient when there are few cells to waste compensating before the data can be acquired. This is efficient when there are few cells to waste compensating before the data can be acquired.

With a single click on a population, the snap-to gate will automatically gate the population. With a single click on a population, the snap-to gate will automatically gate the population. The gate will adjust as the population moves or when toggling between files. The gate will adjust as the population moves or when toggling between files. Snap-To Gating

Flow Cytometry Core Facility To set up time for a demonstration, analysis or training please contact Dr. Phil Streeter or Miranda Boyd. Also, feel free to stop by the facility in the VA Research Center Building 103 room E147. Philip R. Streeter Ph.D. (503) Miranda Boyd (503) x56874