Can Expression of VACM-1 Enhance Effects of Thalidomide on Endothelial Cell Growth? Drake Harper Zeeland East High School John Pelton Maria Burnatowska-Hledin.

Slides:

Advertisements

Similar presentations

VACM-1! By Michael Hledin and Gabe Marquez. Introduction VACM-1 stands for Vasopressin-Activated Calcium Mobilizing Receptor AKA Cullin5 It encodes a.

Advertisements

Figure 13.2 The Biology of Cancer (© Garland Science 2007) Hodgkins Lymphoma – A Cancer in Which 99% of the Tumor Cells Are Not Cancer Cells.

What is Cancer? How it occurs and cell cycle regulation.

ANTIANGIOGENESIS Anticancer Therapy By: Laura Roberts.

Tumor Supressor Gene Non-functional TSG Mutations increasing risk of cancer “Loss of function” mutation Proto-oncogene Oncogene (Hyperactive or unregulated.

Thalidomide Thalidomide – the sleeping pill Thalidomide was first synthesized in West Germany in 1953 by Chemie Grünenthal. It was hailed as a "wonder.

Presented By: Lana Awad and Sebastian Lukjan. Motivation of research, why they did what they did…  Understand steps that cancer cells take to spread.

C. HENDRICKS, L. JANSSEN, R. DELCOMBEL, J. DUBAIL, C. DEROANNE, A. COLIGE Laboratory of Connective Tissues Biology, GIGA-Cancer, University of Liège, Tour.

1. Cancer and the cell cycle

B. Signal Transduction Pathway (cell signaling)

Introduction Angiogenesis is the formation of new blood cells that sprout from pre-existing ones. Essential for a tumor to grow larger then 1-2 mm, which.

Angiogenesis ↓ Metastasis. Angiogenesis--- The process of developing new blood vessels. Cancer cells (probably like all tissues) secrete substances that.

16kDa Prolactin Fragment Inhibits VEGF-induced Ras Activation Through Decreased p62 Dok Phosphorylation A 16-kDalton fragment of prolactin (16Prl) has.

Control of Gene Activity Chapter 17. Controlling gene activity Remember to control the cell one must control protein synthesis. Remember to control the.

Characterization of the anti-angiogenic properties of arresten,an α1β1 integrin- dependent collagen-derived tumor suppressor E xperimental Cell Research.

Isosteviol derivatives induced apoptosis in Human lung cancer via targeting MEK/MAPK pathway: An in vitro and in vivo study Ahmed M Malki 1,,PhD Stephen.

Quantification and DNA Sequencing of IL-13Rα1 and IL-13Rα2 On Various Cancer Cell Lines Erin Dolac, Department of.

Characteristics of Cancer. Promotion (reversible) Initiation (irreversible) malignant metastases More mutations Progression (irreversible)

Infectivity Enhanced Adenovirus as a Strategy for Improving the Efficiency of RNA Interference in an Ovarian Cancer Model T Michael Numnum, MD International.

BioH Chapter 14 – Control over Genes. Control of Gene Expression Cells are selective about which genes they require This depends upon:  Cell type  Specific.

Computational biology of cancer cell pathways Modelling of cancer cell function and response to therapy.

SP Cancer Metastasis Summary Hypothesis: We hypothesize that miRNAs regulate breast cancer cell invasiveness and metastasis by synergistically targeting.

Km23: a Novel Protein in the TGF  Signaling Pathway Nicole Dague Department of Biological Sciences, York College of Pennsylvania ABSTRACT An innovative.

What controls cell growth Objective: To study limits on cell growth and why cancer occurs in cells.

P53 Missense Mutation Cancer. Outline Disease related to p53 Role and regulation pathway Structure of p53 Missense mutation and consequences Experiment’s.

Essentials of Biology Sylvia S. Mader

Chapter 12: The Cell Cycle

Video Homework Assignment “Cell Biology & Cancer” Reference Pgs: Chapter 12: pgs Chapter 19: pgs

Cell Communication Chapter Cell Communication: An Overview  Cells communicate with one another through Direct channels of communication Specific.

I.Cancer Cancer cells evolve and accumulate traits that distinguish them from normal cells causing them to form tumors cancer cellnormal cell Matthew Saelzler.

Fibroblast Growth Factors (FGFs)

MITOSIS: CELL CYCLE CONTROL. OVERVIEW: Has 4 phases – G 1, S, G 2, and M Driven by specific molecular signals present in the cytoplasm Controlled by a.

 The timing and rate of cell division is crucial to normal growth, development, and maintenance of multicellular organisms.

Relationship Between STAT3 Inhibition and the Presence of p53 on Cyclin D1 Gene Expression in Human Breast Cancer Cell Lines Introduction STAT3 and p53.

Gene Expression Chapter 16. DNA regulatory sequence All on DNA Promoters – Start transcription Promoters – Start transcription Terminators – End Transcription.

TUMOR ANGIOGENESIS. Formation of new blood vessels is called angiogenesis Tumor angiogenesis is the proliferation of a network of blood vessels that penetrates.

Regulating The Cell Cycle. Warm Up – The Cell Cycle The cell spends 80% of the time in _______________ and 20% of the time in ________________ What are.

Date of download: 6/22/2016 Copyright © The American College of Cardiology. All rights reserved. From: An Endocrine Genetic Signal Between Blood Cells.

Products > MCF-7 Transfection Reagent (Breast Cancer, HTB-22) Altogen Biosystems offers the MCF-7 Transfection Reagent among a host of 100+ cell line specific.

Speaker: Kacy Richmond Advisor: Dr. Jun Ren (School of Pharmacy)

Student: Nathan King Professor: Dr. Dae-Sik Lim Project Supervisor: Minchul Kim Activity of Merlin (NF-2) in Novel Tumor Pathway.

Products > HUVEC Transfection Reagent (Human Umbilical Vein Endothelial Cells) Altogen Biosystems offers the HUVEC Transfection Reagent among a host of.

Companion PowerPoint slide set Obesity-associated breast cancer risk: a role for epigenetics? This teacher slide set was created by Dana Haine, MS, of.

A novel bispecific ligand-directed toxin designed to simultaneously target EGFR on human glioblastoma cells and uPAR on tumor neovasculature Brandon Kats.

ANIMAL CELL CULTURE.

MicroRNA-101 Inhibits Growth, Proliferation and Migration and Induces Apoptosis of Breast Cancer Cells by Targeting Sex-Determining Region Y-Box 2 Cell.

Inducing Angiogenesis

Activating Invasion and Metastasis

Slit-Robo Cancer Cell Volume 4, Issue 1, Pages 1-2 (July 2003)

Volume 14, Issue 5, Pages (November 2008)

Overexpression of ornithine decarboxylase enhances endothelial proliferation by suppressing endostatin expression by Takahiro Nemoto, Hisae Hori, Masataka.

Chapter 12: The Cell Cycle

Chapter 12: The Cell Cycle

Chapter 12: The Cell Cycle

Combining the Multitargeted Tyrosine Kinase Inhibitor Vandetanib with the Antiestrogen Fulvestrant Enhances Its Antitumor Effect in Non-small Cell Lung.

Expression and regulation of Toll-like receptor 2 by IL-1β and fibronectin fragments in human articular chondrocytes S.-L. Su, M.S., C.-D. Tsai, Ph.D.,

Sarah Otih Mentor: Dr. William Barton

Sarah Otih Mentor: Dr. William Barton

10.3 Regulating the Cell Cycle

TGF-β Receptor I/ ALK-5 and Pancreatic and Biliary Cancer

AP Biology The Cell Cycle.

Volume 127, Issue 4, Pages (October 2004)

Chapter 12: The Cell Cycle

Chapter 12: The Cell Cycle

Regulating the Cell Cycle

(Top) Micrographs of SNAIL-HA and mock-infected cells.

EGFR and cetuximab sensitivity of SCCUAT

Activation of mTOR signaling (increased phosphorylated S6 ribosomal protein) and loss of inhibition of IRS-1 in human endometrial carcinomas. Activation.

Effect of SFN on the total activity and protein expression of HDACs in JB6 P+ cells. Effect of SFN on the total activity and protein expression of HDACs.

Presentation transcript:

Can Expression of VACM-1 Enhance Effects of Thalidomide on Endothelial Cell Growth? Drake Harper Zeeland East High School John Pelton Maria Burnatowska-Hledin Hope College

VACM-1 (Vasopressin-Activated Ca 2+ -mobilizing receptor) Isolated and studied by Hledin’s Lab Inhibits Cell growth by regulating cAMP and MAPK phosphorylation, and p53 concentration, signaling pathways involved in cell growth that lead to cancer In vivo VACM-1 is expressed in endothelial cells (cells in vasculature) Mutation in VACM-1 sequence (where one amino acid is changed) creates a dominant negative phenotype (reverse the effect) ie. Cell growth increases

Thalidomide Originally created to help pregnant women with “morning sickness” Caused Birth Defects (Physical deformities on limbs and organs), Later banned Reason for Study: Inhibits Angiogenesis (the growth of blood vessels) Cellular targets for Thalidomide’s effects are unknown

Hypothesis In normal endothelial cells expressing VACM- 1 protein, thalidomide will decrease cell growth by inducing VACM-1 protein expression. In cells expressing a dominant negative VACM-1 (VACM-S730A), Thalidomide will increase the mutated VACM-1 but will not inhibit growth.

Uses on cancer VACM-1 stops cell growth, therefore, if used on cancer cells, it should stop the growth of cancerous tumors on cells.

Methods Cell Culture Western Blot Wound Healing Assay Immunostaining

Western Blot GAPDH

Data

Summary Thalidomide increases VACM-1 protein concentration which results in decreased growth. When VACM-1 is mutated, it is unable to inhibit cell growth Conclusion Anticancer effect of Thalidomide may involve induction of VACM-1

Acknowledgements Dr. Hledin John Pelton Dr. Hledin’s lab group REACH Hope College