V. Microbiology of water V. Microbiology of water A. Waterborne microbial pathogens B. Indicator bacteria for drinking water C. Other indicators for drinking.

Slides:



Advertisements
Similar presentations
Recreational Water Sampling. References A.Guidelines for Canadian Recreational Water Quality by Health & Welfare Canada 1992 B.CFP 213 CF Health Manual.
Advertisements

Indicator Microorganisms – Chapter 23
Drinking Water Treatment – Chapter 25 Class Objectives Be able to define the possible components of a water treatment train and their functions Be able.
Introduction to Environmental Engineering Lecture 14 Water Quality Continued Chapter 8.
Water Investigation Unit: Background Information.
Jeanette A. Thurston-Enriquez
Microbiological Examination of Water January 17, 2007 Dr. Paul F. Vendrell.
Environmental Biotechnology CE421/521 Tim Ellis October 25, 2007.
Surface Water Treatment Rule Bob Clement Drinking Water Program U.S. EPA Region 8.
Fermentation Test (Phenol Red). John Snow’s Cholera Spot Map.
2-2 Microorganism Small, living organism Pathogen Disease-causing microorganism Toxin Poison Spoilage Microorganism Microorganism that causes spoilage,
6/2/ General Water Microbiology Robin Cook FSEA Fall Meeting and Technical Session 10/17/2007.
WaterBy: Andrew Paek Nirav Parekh. Background  Water is an essential part of our well being  We use it for nourishment, cooking, cleansing, and many.
Water and Infectious Disease - Waterborne Disease Global distribution of infectious disease Transmission cycles Water and infectious disease Enteric disease.
Agricultural & Environmental Lab. Water quality testing II: PCR-based testing for water bacterial contaminants The Islamic University Faculty of Science.
Lecture 2 Microbial Indicators of Fecal and Other Types of Environmental Contamination ENVR 133 Mark D. Sobsey.
1 Microbial Pathogens n Living organisms that cause disease –Can be n Viruses n Bacteria n Protozoa n Helminths –But not all are pathogens.
Biodegradation of Chemicals Natural organics typically degraded quickly Most xenobiotics not as easily broken down –Synthetic chemicals are often recalcitrant.
Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings PowerPoint ® Lecture Slide Presentation prepared by Christine L. Case Microbiology.
Copyright © 2006 Pearson Education, Inc., publishing as Benjamin Cummings PowerPoint ® Lecture Slide Presentation prepared by Christine L. Case M I C R.
Coliform organisms Elvire Jacques, MD Environmental health.
Chapter 4 Microbiological Contaminants
Presence of Microbial Indicators in Reid Park Wetlands Jepson Sutton Scott Stine SWES 574.
Waterborne Pathogens: Bacteria February 9 th -11 th, 2010.
Microorganisms (The Coliform Group Bacteria) S. D. Spence.
Microbiology: Testing for Bacteria Linda Wolf Glencoe High School SWRP Teacher for 12 years.
Coliform Bacteria in Water
Introduction to Lab Ex. 20: Enumeration of Bacteria - Most Probable Number method Membrane Filter method.
Measuring Stream Microbiology: Methods and Preliminary Results Dr. Robert B. Simon Mr. Jonah Stevens Department of Biology SUNY-Geneseo.
Water Microbiology Tap (drinking) water Surface water (freshwater) Waste water (Marine water) Water borne diseases.
Measuring Stream Microbiology:
BIO 411 – Medical Microbiology Chapter 9 Commensal and Pathogenic Microbial Flora.
Ch.3.  Potential health effects from ingestion of water Radionuclides: Increased risk of cancer, kidney toxicity. Erosion of natural deposits of certain.
© 2013 Pearson Education, Inc. Chapter 25: Microbial Diseases of the Digestive System $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Structure.
Water Microbiology I MPN test.
Lab 10 BACTERIOLOGY OF THE GASTROINTESTINAL TRACT Lab Manual (p.67-82)
Manaaki Tangata Taiao Hoki protecting people and their environment through science Specialist Science Solutions Water microbiology Beware of the little.
Applied Environmental Microbiology 43 Copyright © McGraw-Hill Global Education Holdings, LLC. Permission required for reproduction or display.
Water Purification Chemistry.
Hillsborough River Fecal Coliform BMAP Process Oct. 22, 2008.
FST 304 FOOD MICROBIOLOGY By Prof. Olusola Oyewole And Dr. Olusegun Obadina.
WATERBORNE INFECTIOUS DISEASES David L. Taylor, PhD Infection Preventionist Dept of Clinical Epidemiology The Ohio State University Medical Center.
Lab 29 Water labs.
MONITORING THREATENED SOUTHERN SEA OTTERS FOR EXPOSURE TO SELECTED FECAL PATHOGENS Melissa A. Miller, Nancy Christian, Mike Murray, Dave A. Jessup Melissa.
Environmental Microbiology (MLEM-201) What do Microbes do? How can we use this to our advantage? Lecturer: Dr. Mohamed Salah El-Din.
The Gram Negative Bacilli Family Enterobacteriaceae
© 2008 Awwa Research Foundation. ALL RIGHTS RESERVED. MICROBIAL MYTHS Martin J. Allen, Ph.D © 2008 Awwa Research Foundation. ALL RIGHTS RESERVED. No part.
Introduction All pathogenic microorganisms implicated in foodborne diseases are considered enteric pathogens, except S. aureus, B. cereus, C. botulinum.
Coliforms
Waterborne Diseases Water Quality Coliform Counts Lactose + gas Extinction Dilution Most Probable Numbers – MPN EMB agar.
Microbiological Measurements Waterborne Pathogens Salmonella (typhoid fever) Shigella (bacillary dysentery) Hepatitus virus Entameoba Histolytica (ameobic.
Copyright © The McGraw-Hill Companies. Permission required for reproduction or display. 1 Applied Environmental Microbiology Chapter 35.
利用最大可能數(most probable number, MPN)測定水樣品大腸菌群數
Chapter 20 Indicators of Foods Microbial Quality and Safety Tsuei-Yun Fang 節錄自 Modern Food Microbiology, 2005Jay, J. M., 7th ed.
M. A. El-Farrash. Water is the most important substance in our daily life. Safe drinking water is essential to humans and other life forms, as water.
Microorganisms (The Coliform Group Bacteria)
利用最大可能數(most probable number, MPN)測定水樣品大腸菌群數
BTEC 223 Lab Exercise Water Module
Water Quality & micro-organisms
Practical 5 Water Microbiology I
Introduction to detection methods
The Need for Chlorine.
Microbiology of Water Dr.GulveR.M.
Microbiology of Water & Air
Microbial Diseases of the Digestive System
Applied Environmental Microbiology
Environmental Biology
Fate and transport of pathogens in water
Rapid diagnosis of gastrointestinal pathogens
WATER SERVICES FORUM WATER QUALITY
Presentation transcript:

V. Microbiology of water V. Microbiology of water A. Waterborne microbial pathogens B. Indicator bacteria for drinking water C. Other indicators for drinking and recreational water

A.Waterborne microbial pathogens Microbiology review: u Microbes in water include: –Bacteria –Virus –Protozoa u A few microbes (pathogens) are capable of causing disease, and may be transmitted by water.

Waterborne pathogens: u Some common pathogens: u Salmonella typhi u Escherichia coli u Vibrio cholera u Pseudomonas aeruginosa u Shigella spp. u Cryptosporidium u Giardia lamblia u Norwalkvirus

Cryptosporidum and cryptosporidiosis Cryptosporidium parvum Crypto “Oocysts

Giardia lamblia (giardiasis) Original image by Arturo Gonzalez, CINVESTAV, Mexico. Used with permission of Javier Ambrosio, UNAM, Mexico

Giardia lamblia u Giardia lamblia trophozoites live in the small intestine of the host. u The trophozoites average about 15 µm in length, have a distinct "tear-drop" shape and two nuclei at the anterior end Giardia lamblia troph. The two nuclei are easy to see in this image. (Original image from a Japanese language site tentatively titled "Internet Atlas of Human Parasitology."

B. Indicator bacteria for Drinking Water: u Indicator microorganisms are used to indicate an increased risk of pathogen contamination due to fecal contamination. u Indicator microbes should be: –Always present when feces/sewage is present –Always absent when feces/sewage is absent –Survives longer in water than any of the pathogenic species –Easily isolated and identified.

Indicator bacteria: u Coliform bacteria (E. coli-like) are the most often used indicator bacteria for water quality assessment in the U.S.

Characteristics of coliforms: u Aerobic or facultative, u Gram-negative, u Non-spore forming, u Bacilli, u which ferment lactose to form acid and/or gas within 48 hours at 35 o C.

More specific coliforms: u Coliforms are often found naturally in soil, water, plants, etc. u Fecal coliforms are a more specific coliforms that usually come from feces. u E. coli is the most specific indicator

Guidelines and methods for enumerating coliforms: u EPA guidelines for coliforms in drinking water are < 1 CFU/100 ml. u Tests used to isolate and enumerate coliforms in water include: –m-Endo media –m-FC media –MUG media

C. Other indicators for drinking and recreational water C. Other indicators for drinking and recreational water u Limitation of Total coliform as drinking water microbial indicator –Ubiquitous. –Less resistant to traditional disinfection. –Proliferate in the biofilms of water distribution systems

u Limitation of Fecal coliforms –Klebsiella pneumoniae –Less resistant to traditional disinfection. u Limitation of E. coli –Less resistant to traditional disinfection.

Microbial Indicators for Recreational Water u Total coliform? u Fecal coliform? –Average 200 MPN/100 ml u E. Coli (fresh water)? –Average 126 MPN/100 ml. u Enterococci (salt water) –Average 35 MPN/100 ml.