Possible factors affecting ribosomal protein expression 1.Autogenous regulation of protein expression. 2.mRNA protein degradation. 3.mRNA secondary structure.

Slides:

Advertisements

Similar presentations

20,000 GENES IN HUMAN GENOME; WHAT WOULD HAPPEN IF ALL THESE GENES WERE EXPRESSED IN EVERY CELL IN YOUR BODY? WHAT WOULD HAPPEN IF THEY WERE EXPRESSED.

Advertisements

Molecular Genetics DNA RNA Protein Phenotype Genome Gene

©2001 Timothy G. Standish Psalm 102:25 25In the beginning you laid the foundations of the earth, and the heavens are the work of your hands.

Methylation, Acetylation and Epigenetics

Gene Regulation. Levels of Regulation DNA rearrangement –Immune System rearranges DNA Bacteria can change DNA in chromosomes Not as common as other methods.

Intro to Molecular Genetics RNA & Protein Synthesis 3/16/2011.

Chapter 22 (Part 2) Protein Synthesis. Ribosomes.

Chapter 15 Noncoding RNAs. You Must Know The role of noncoding RNAs in control of cellular functions.

Chapter 19: Eukaryotic Genomes Most gene expression regulated through transcription/chromatin structure Most gene expression regulated through transcription/chromatin.

AP Biology Control of Eukaryotic Genes.

Regulation of Gene Expression Eukaryotes

AP Biology Control of Eukaryotic Genes.

Transcription & Translation Transcription DNA is used to make a single strand of RNA that is complementary to the DNA base pairs. The enzyme used is.

DNA encodes messenger RNA

Gene Regulation II : The Ribosome Strikes Back!. Mechanisms Covered Attenuation Control –Tryptophan Biosynthesis Riboswitches –Tryptophan Biosynthesis.

Controlling Gene Expression. Control Mechanisms Determine when to make more proteins and when to stop making more Cell has mechanisms to control transcription.

Biology 102 Gene Regulation and Expression Part 2.

©2000 Timothy G. Standish Hebrews 1:1-2 1God, who at sundry times and in divers manners spake in time past unto the fathers by the prophets, 2Hath in these.

RNA Makin’ Proteins DNAMutations Show off those Genes!

Genetic Transformation of Bacteria and Gene Regulation.

CFE Higher Biology DNA and the Genome Transcription.

Part 5 Translation Translation: mRNA and the Genetic Code Triplet code- codon 3 sequential Nitrogen bases in mRNA 64 different mRNA codons.

Regulating Gene Expression To accompany “Regulating Gene Expression” Packet -review packet reading, pictures, and questions.

Aim: How is DNA organized in a eukaryotic cell?. Why is the control of gene expression more complex in eukaryotes than prokaryotes ? Eukaryotes have:

Gene Expression II. Translation Overview Conversion of triplet code into polypeptide Takes place at ribosome in cytoplasm Involves all 3 types of RNA.

Recombinant DNA Technology In Vitro TRANCRIPTION AND TRANSLATION

(3) Gene Expression Gene Expression (A) What is Gene Expression?

Information flow from DNA to trait

Protein Synthesis Molecular Biology

RNA and protein synthesis

(3) Gene Expression Gene Expression (A) What is Gene Expression?

Organization and control of Eukaryotic chromosomes

Protein Synthesis Part 3

B.Ed. Secondary Science Education

NOTES: RNA & Protein Synthesis Overview

Cytosol Nucleus Mitochondria Secreted ER Golgi Endosome Lysosome Transmembrane.

Genetic Transformation of Bacteria and Gene Regulation

Gene Expression Continued

Transcription and Gene Regulation

Project Studying Synechococcus elongatus for biophotovoltaics.

Protein Synthesis Part 3

Control of Gene Expression

محاضرة عامة التقنيات الحيوية (هندسة الجينات .. مبادئ وتطبيقات)

Regulation of Gene Expression

Cell Division and Gene Expression

Daily Warm-Up Thursday, January 9th

Protein Synthesis Part 3

Figure 2 Functional mechanisms of ASOs

mRNA attaches to a Ribosome

Eukaryote Regulation and Gene Expression

Central Dogma Translation

Knowledge of a gene or it’s gene product is necessary and essential

Suppl.Figure 1 Transfected HEK293 cells -130kDa -100KDa Blot: -V5

RNA and Protein Synthesis

Protein Synthesis Lecture 5

Lesson 1: Evolution.

Protein Synthesis Miss Richardson SBI4U.

Monday’s Writing Topic ~ Week 20 – January 25 – 29 ~

Welcome to the PROTEIN SHOW! T A G C With your host, RNA POLYMERASE!

RNA and protein synthesis

The Noncoding RNA Revolution—Trashing Old Rules to Forge New Ones

Structure of the Genome

Chapter 10 – The Gene and Protein Synthesis

From gene to protein.

Translation converts an mRNA message into a polypeptide, or protein.

Proteins: Building Blocks of Life

2/28/12 Objective: Understand how the structure of tRNA makes translation possible Warm-Up: How does the genetic code work? Homework: Simulating Protein.

Presentation transcript:

Possible factors affecting ribosomal protein expression 1.Autogenous regulation of protein expression. 2.mRNA protein degradation. 3.mRNA secondary structure. 4.Missing factors in E.coli S-30 extract

S1 S2 S4 S5 S13? S16? S?

“Specialized” ribosome GFP X W X W Ribosome N N S N AGUGTAU UCACAUA

Future Steps and Issues 1.Increase translation efficiency. 2.Reconstitute 30S and 50S subunits. 3.Genome organization. 4.Autogenous regulation. 4. RNA processing & modifications.