Cytoskeleton IV Chapter 16. Mechanochemical cycle of myosin.

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Presentation transcript:

Cytoskeleton IV Chapter 16

Mechanochemical cycle of myosin

DVD clip 74

Striated vs. smooth muscle cells

Thick and thin filaments in smooth muscle cells

Skeletal muscle cells form by the fusion of many muscle cell precursors called myoblasts 50 micrometers wide, several cm long

Skeletal muscle cells in rats showing blue stained nuclei

Skeletal Muscle Myofibrils

Closeup of two sarcomeres

Schematic diagram showing components of dark and light bands

Insect flight muscle viewed in cross section show myosin and actin packed together

Sliding filament model of muscle contraction

Accessory proteins in a sarcomere

Ion Channels at Neuromuscular Junction

Ca2+ release channel is opened by transmembrane protein in tubule

Two membrane system that relays signal to contract muscle cells

Electron micrograph showing 2 T tubules

Muscle contraction is controlled by troponin troponin can pull tropomyosin of myosin binding sites

Ca 2+ relieves tropomyosin blockage

Cell Cycle and Programmed Cell Death Chapter 17

The cell cycle can be studied in a cell free system

The cell cycle is often studied in immortalized mammalian cell lines

Activation of a cyclin dependent kinase

CdK activity can be suppressed by inhibitory phosphorylation

CdK activity can be suppressed by inhibitory proteins

Cell fusion experiments

Assembly of pre-replicative complex during G1 phase

Entry into mitosis is blocked by incomplete DNA replication Hydroxyurea blocks DNA synthesis Caffeine causes failure of checkpoint

The Rb protein acts as a brake in mammalian cells

Overview of cell cycle control system