Immunology Overview
Innate vs Acquired Immunity Non-specific, rapid Physical, Chemical Barriers Complex Biochemical Mechanisms –Fever –Inflammation –Complement –Toll and Nod Antigen specific and initially slow Memory Lymphocytes Humoral and Cellular Exquisitely Specific Recognition molecules
Eukaryote Innate Immunity
Cells of the Immune System
The Immune System
The Clonal Selection Theory
Primary vs Secondary Response
Antigenicity Antigen - any molecule that binds to and activates the specific receptors of lymphocytes –Immunogenicity –Specificity Epitopes Some molecules (haptens) have specificity but no immunogenicity -- these need a carrier –Digoxigenin –peptides
DIG - KLH
Requirements for Immunogenicity Foreignness High Molecular Weight Chemical Complexity Degradability Host Factors –Genetics –Route of encounter / Adjuvants
Protein Structure
Myoglobin Epitopes
Lysozyme Epitopes
Compare Epitope Structures
Antibody Structure
The 5 Antibody Classes
Mammalian Antibody Isotypes
The Immunoglobulin Superfamily
The Central Dogma
and GOD said, “Let there be light chains!”
Light chain exon rearrangement
“and let there be heavy chains!”
The Clonal Selection Theory
Monoclonal Antibodies