IGF-1 GENE EXPRESSION: an instantaneous growth indicator for tilapia Emmanuel Vera Cruz, Christopher Brown, Remedios Bolivar, Russell Borski, students, and colleagues Emmanuel Vera Cruz, Christopher Brown, Remedios Bolivar, Russell Borski, students, and colleagues Cloning, diet (2006) Aquaculture 251: Cloning, diet (2006) Aquaculture 251: Social Status (2007) Hormones and Behavior. 51: Social Status (2007) Hormones and Behavior. 51: Temperature dependence, in press Temperature dependence, in press Influence of photoperiod, in press Influence of photoperiod, in press

Isolation of IGF-1 mRNA, Oreochromis niloticus 1 TTCTCCAAAACGAGCCTGCGCAATGGAACAAAGTCGGAATATTGAGATGTGACATCGCC CGCATCTCATCCTCTTTCTCCCTGTTTTAATGACTTTAAACAAGTTCATTTTCGTCGGGC 119 Signal Peptide ( ) 120 TTTGTCTTGTGGAGACCCGTGGGGATGTCTAGCGCTTTTTCCCTTCAGTGGCATTTATGT 179 M S S A F S L Q W H L C 180 GATGTCTTCAAGAGTGCGATGTGCTGTATCTCCTGTAGCCACACCCTCTCACTACTGCCG 239 D V F K S A M C C I S C S H T L S L L P B domain( ) 240 TGCGTCCTCACCCTGACTCCGACGGCAACAGGGGCGGGCCCTGAGACCCTGTGCGGGGCG 299 C V L T L T P T A T G A G P E T L C G A 300 GAGCTGGTCGACACGCTGCAGTTTGTCTGTGGAGAGCGAGGCTTTTATTTCAATAAACCA 359 E L V D T L Q F V C G E R G F Y F N K P C domain ( ) A domain ( ) 360 ACAGGCTATGGCCCCAGTGCAAGGCGGTCACGTGGCATCGTGGACGAGTGCTGCTTCCAA 419 T G Y G P S A R R S R G I V D E C C F Q D domain ( ) 420 AGCTGTGAGCTGCAGCGCCTTGAGATGTACTGTGCACCTGTCAAGACTCCCAAGATTTCT 479 S C E L Q R L E M Y C A P V K T P K I S E domain ( ) 480 CGCTCTGTGCGTTCACAGCGGCACACAGACATGCCAAGAGCACCCAAGGTTAGTAGCAGA 539 Close (84-90%) sequence identity with other IGF-1s

Hepatic gene expression as related to weight gain

Temperature-dependent differences in weight gain and IGF-1 expression

Effect of environmental temperature on IGF-1 expression

Social dominance and IGF-1 expression

Correlation of aggression w/ IGF-1 expression

Photoperiod-induced changes, growth & IGF-1

Temperature influence on growth rate

Correlative change in IGF-1 expression…

… resulting from tight association of growth with IGF-1 expression

Acknowledgements Funding agencies Funding agencies USAID/PDA CRSP USAID/PDA CRSP The Fulbright Foundation The Fulbright Foundation Institutions Institutions Central Luzon State University Central Luzon State University North Carolina State University North Carolina State University Florida International University Florida International University NOAA, The Milford Laboratory NOAA, The Milford Laboratory

Thank you!