Nasonia vitripenni You are the PI ( P rimary I nvestigator)

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Presentation transcript:

Nasonia vitripenni You are the PI ( P rimary I nvestigator)

Jewel Wasp Tell me about it…

Life Cycles

Haplo/Diplo Genetics

Wasp Development Timelines 18 o, 28 o C

Complementation Process by which an allele of each of two mutant genes makes up for the defect in the other gene. Process by which an allele of each of two mutant genes makes up for the defect in the other gene.

You Find Three Mutants With a Similar Phenotype Are they the Same Genotype?

Possible Physiology Enzyme A Enzyme B Enzyme C Enzyme A Enzyme B Enzyme C Enzyme A Enzyme B Enzyme C Enzyme A Enzyme B Enzyme C

Complement a||Ba||B a||Ba||B A||bA||b A||bA||b x A||bA||b a||Ba||B Two genes

Fail to Complement A||bA||b A||bA||b A||bA||b A||bA||b x A||bA||b A||bA||b 1 gene

Three scarlet eyed mutants, how many loci? st DR, st C, st 318 Connect same gene with a line. st DR st 318 st C Fail to complement For example.

Handling Wasps

Sexing Wasps adults

Sexing Wasps larvae, tuxedo – black stages

Sex Pictures

Scoring Red Eyes Here

Scoring Eye Color

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To Do Plan experiment! Plan experiment!  define symbols, diagram crosses, determine expectations, etc. Each student : complementation. Each student : complementation. Per bench, or per pair (will tell tomorrow), Per bench, or per pair (will tell tomorrow),  recessive, dominance,  controls (stock maintenance). Wednesday

st DR, st C, st 318 F1 data, F2 data that means something

Friday Tinker with wasps, Tinker with wasps, aha3-1 segregation analysis. aha3-1 segregation analysis. Plant Arabidopsis in soil, Plant Arabidopsis in soil, Introduction to Mitochondrial DNA, maternal genotyping, Introduction to Mitochondrial DNA, maternal genotyping, Basic Mendal? Basic Mendal?