Also known as wet chemistry and bench chemistry Handling Liquids Robyt and White2.1-2.3; pp. 21-27 Also known as wet chemistry and bench chemistry

Water Type of water Contaminants Removed Applications Tap Coliforms, Solids, Poisons Washing Dishware Tap Distilled (dH2O) Most Minerals (“hard water”) Rinsing Dishes Media Glass Distilled (gdH2O; ddH2O) Minerals from pipe Media, Buffers and reagents De-ionized (dIH2O) Trace volatile organics Bicarbonate Molecular Biology

Carboys Hold large quantities 2-20 L Bulk solutions: TAE Buffer, SSC Buffer Remember to crack the lid!

Erlenmeyer Flasks 125 ml -4 liters Melting agar media, agarose Can be heated and autoclaved Not intended for storage

Beakers 100 ml – 2 liters Start solutions, media without agar Can be heated Not intended for storage

Screw-cap Bottles Volumes 100 ml to 1000 ml Cheap, autoclaveable, reusable Molecular Biology reagents, Liquid Media Do not put on hot plate! Storage

Conical Tubes Plastic, sterile, disposable centrifuge tubes 15 ml or 50 ml size Grow bacteria – 5ml media in 50 ml tube Filter-sterilized solutions and media Great for storage (including freezing)

Microcentrifuge Tubes Plastic, sterile, disposable centrifuge tubes 2, 1.5, 0.5, and 0.2 (microamp) formats Most molecular techniques, small reaction volumes Special racks and storage

Graduated Cylinders vs Volumetric Flasks

Pipetters Air-displacement piston pipettes Disposable, sterile, plastic tips Volumes 0.5 ml – 1000 ml

Downstream