Getting Biodiesel From Jathropa The jatropha seeds, commonly known as the physic nuts, contain up to 40% oil. The jatropha oil can be used directly as biofuel or can be converted into biodiesel for a more efficient performance. When raw or mixed jatropha oil is directly used in an automobile or even plane engines

Jatropha genotyping In Gh Pu QR 13 primer pairs selected for use in further studies These reveal 69/453 polymorphic bands (15.2%) Results indicate very little variation between accessions from India, Ghana, Tanzania & Madagascar

Considerations for using DNA markers in plant breeding Technical methodology –simple or complicated? Reliability Degree of polymorphism DNA quality and quantity required Cost** Available resources –Equipment, technical expertise

Genetic Markers A gene or DNA sequence having a known location on a chromosome and associated with a particular gene or trait Advantages of genetic markers: –Profile of genetic markers will define a genotype –Uniform at all tissues at all ages of the organism –allows early detection –developmentally stable and not affected by the environment –highly specific pattern can be developed for each individual Utilities: –For biodiversity study –To choose right parents for cross pollination –Marker assisted breeding –To differentiate plant varieties –Quality control of plantation

RFLP

(1) LEAF TISSUE SAMPLING (2) DNA EXTRACTION (3) PCR (4) GEL ELECTROPHORESIS (5) MARKER ANALYSIS Overview of ‘marker genotyping’