achaete stade 9stade 10 Lsc Achaete Achaete+Scute Gène proneural Groupe proneural.

Slides:

Advertisements

Similar presentations

MCDB 4650 Developmental Genetics in Drosophila

Advertisements

Chapter 21. Development of Multicellular Organisms

Notch receptor activation and function. Lz Notch signaling completely changes the way that cells respond to other signaling pathways Flores et al., Cell.

Next lecture: Induction/Signaling Requirements of inducer and responder cells Cascades of inductive events are involved in forming organs Examples of the.

Neurulation & Organogenesis. Neurulation Neural cells originate from the neurectoderm Clusters of proneural cells are specified by the proneural genes.

Chapter 4 The Emergence of Neural Fate. PNS Neurons in one Abdominal Hemisegment d v’ l v.

Development of the nervous system - I

MCDB 4650 Cell Signaling in Development. Why might a cell (cell 1) activate a transcription factor in response to a growth factor binding an RTK while.

Early L(1)sc expression: Are there L(1)sc equivalence groups? Stage 9 8 L(1)sc + cells Early Stage 10 All posterior cells L(1)sc L(1)sc + cells.

Notch1 and pre-T-cell Acute Lymphoblastic Leukemia (T-ALL) by Lindsey Wilfley.

Early Development: Invertebrates  Brief overview of early developmental themes  Early development of Sea Urchin  Early development of Caenorhabditis.

MCDB 4650 Induction and Mesoderm Patterning. If you isolate animal cap cells from a Xenopus embryo at the 8-cell stage and assay them 1 day later, they.

Genetic models Self-organization How do genetic approaches help to understand development? How can equivalent cells organize themselves into a pattern?

Genetic models Self-organization How do genetic approaches help to understand development? How can equivalent cells organize themselves into a pattern?

Flies are quick!. The fly body plan: each segment has a unique identity and produces distinctive structures 3 head 3 thorax 8 abdomen.

I can’t wait to grow up! Laugh now.

Notch1 and its role in pre T-cell Acute Lymphoblastic Leukemia (ALL) By Rebecca Goodman.

HeadTail T1 T2 T3 A1 A2A4 A5 A6 A7 A3 A8 Metamorphosis Quiltwork Assembly of a Fly.

Building a functional nervous system Immature CNS Cells Motorneurons Neurosecretory cells Glia Interneurons Cell fate determination Cell migration Apoptosis.

Drosophila dorsal/ventral axis detemination How are different tissue types specified at distinct positions on the embryonic dorsal- ventral axis?

Drosophila dorsal/ventral axis detemination

HeadTail T1 T2 T3 A1 A2A4 A5 A6 A7 A3 A8 Metamorphosis Quiltwork Assembly of a Fly.

Embryonic development OvumFertilised ovum Cell Division.

LECT 24 and 25: CELL SIGNAL TRANSDUCTION Cells respond to molecular cues they receive: from neighboring cells or extracellular matrices (paracrine factors),

February 06 Developmental biology: imaginal discs 5 wingless hedgehog/engrailed * after several hours of interdependence of wingless and hedgehog: situation.

The Generation and Survival of Nerve Cells

Drosophila axis detemination; dorsal/ventral polarity How are the embryonic axes set up? How does the embryonic dorsal-ventral axis get translated into.

The C. elegans Germline Stem Cell Niche Hall et al

How do equivalent cells acquire different cell fates?

Figure 5.1 Cell cycles of somatic cells and early blastomeres (Part 1)

Final noon Thursday May 5

Drosophila Development: Embryogenesis

Another Notch on the belt

Appendage development

Notch signalling. Notch signalling. Notch receptors are synthesized as single precursor proteins that are cleaved in the Golgi by a Furin‐like convertase.

Drosophila melanogaster

Model organisms: C. elegans

H3 Dynamics in Wild-Type and fas1-4 Mutant Roots.

Mechanism and Significance of cis-Inhibition in Notch Signalling

Yee-Ming Chan, Yuh Nung Jan Neuron

Eye development: Notch lends a handedness

Gene-Regulatory Interactions in Neural Crest Evolution and Development

A Hedgehog-Insensitive Form of Patched Provides Evidence for Direct Long-Range Morphogen Activity of Sonic Hedgehog in the Neural Tube James Briscoe,

An Invitation to T and More

Volume 9, Issue 3, Pages (February 1999)

Threshold dependent BMP Signaling:

Volume 95, Issue 7, Pages (December 1998)

DFezf/Earmuff Maintains the Restricted Developmental Potential of Intermediate Neural Progenitors in Drosophila Mo Weng, Krista L. Golden, Cheng-Yu Lee

Notch Signaling: The Core Pathway and Its Posttranslational Regulation

Rongwen Xi, Jennifer R. McGregor, Douglas A. Harrison

How do equivalent cells acquire different cell fates?

Volume 11, Issue 22, Pages (November 2001)

Short-Range Cell Interactions and Cell Survival in the Drosophila Wing

A Role of Receptor Notch in Ligand cis-Inhibition in Drosophila

Yee-Ming Chan, Yuh Nung Jan Neuron

Neuronal specification: Notch signals Kuz it's cleaved

Chapter 16 Cell Communication.

Notch and the Immune System

The sterol-sensing domain of Patched protein seems to control Smoothened activity through Patched vesicular trafficking Verónica Martı́n, Graciela Carrillo,

Nuclear Access and Action of Notch In Vivo

Fig. 3 Conditionally expressed CAR using Notch as a signal induction and response pathway system. Conditionally expressed CAR using Notch as a signal induction.

Numb Antagonizes Notch Signaling to Specify Sibling Neuron Cell Fates

Another niche for Notch

Lineage compartments in Drosophila

Volume 86, Issue 3, Pages (August 1996)

Transducing the Dpp Morphogen Gradient in the Wing of Drosophila

Proneural enhancement by Notch overcomes Suppressor-of-Hairless repressor function in the developing Drosophila eye Yanxia Li, Nicholas E Baker Current.

Hernán López-Schier, Daniel St Johnston Developmental Cell

N1 decoys differently inhibit ligand-induced NOTCH signaling.

Presentation transcript:

achaete stade 9stade 10 Lsc Achaete Achaete+Scute Gène proneural Groupe proneural

Type normal Mutant iroquois

C14

Prepattern genes: iroquois etc... Proneural genes: scute etc... Precursor genes: neu, poxn Lineage genes: numb etc... Compartment genes: en, dpp, ap… Lateral inhibition genes: Notch Delta etc... Maternal genes Gap genes Pair-rule genes

wild-typeNotch Crible F2

wild-typeNotch

wild-type Notch achaete

Delta Notch DSL EGF-like LNR Ankyrin

S2 + Notch + Delta aggrégation

Cell autonomy of Notch and Delta wild-type/ wild-type wild-type/ Notch wild-type/ Delta Cell autonomous Cell non-autonomous Heitzler and Simpson, 1991

Behaviour of mutant and wild-type cells along clonal borders wild-type/ wild-type 50%-50% Reduced Delta/ wild-type wild-type/ Increased Delta Reduced Notch/ wild-type wild-type/ Increased Notch 0%-100% 100%-0% Cells with a higher level of Notch or a lower level of Delta are more likely to adopt the epidermal fate Heitzler and Simpson, 1991

Notch Notch act Artavanis-Tsakonas, Struhl, Young, 1993

Receiving cell Notch Extracellular P2: TACE/Kuzbanian Delta 2. Ligand-induced S2 processing Signaling cell

Receiving cell Notch CSL HES nucleus Su(H) E(spl) nucleus 4. Nuclear import and transcriptional activation

Gènes cibles et boucles de retro-contrôle E(spl) Notch - intra Mastermind Su(H) Achaete-Scute Complex genes Delta E(spl)

NotchDelta AS-C

NotchDelta AS-C prepattern

NotchDelta AS-C fluctuations aléatoires

NotchDelta AS-C Neuroblaste (100%) Epidermoblaste

Génes de prepattern: iroquois etc... Gènes proneuraux: scute etc... Gènes des précurseurs: neu, poxn Gènes du lignage: numb etc... Génes cardinaux: en, dpp, ap, wg C25