Most proteins in the ER are glycosylated Proteins in cytosol are rarely glycosylated original precursor oligosaccharide added to most proteins in the ER.

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Presentation transcript:

Most proteins in the ER are glycosylated Proteins in cytosol are rarely glycosylated original precursor oligosaccharide added to most proteins in the ER

Protein glycosylation in the rough ER

Oligosaccharides are used as tags to mark the state of protein folding

Misfolded proteins are exported from ER and destroyed

Chapter 13 Intracellular Vesicular Traffic

Roadmap of protein traffic inside cell

Endocytic and secretory pathways red = secretory green = endocytic blue = recycling

5.14 Figure 5.14 figure jpg

Vesicular transport

There are various types of coated vesicles

Different coats are used for different transport steps in the cell

Coated Pits are the sites of receptor mediated endocytosis Clathrin protein cytosol Material to be taken up by cell

Clathrin-coated pits and vesicles

Structure clathrin triskelion

Arrangement of triskelion on surface of clathrin coated vesicle

DVD Clip 57

Assembly and disassembly of clathrin coat

Dynamin pinches clathrin coated vesicles from the membrane

Electron micrograph from Drosophila mutant

Resting Chemical Synapse

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SNARE proteins guide vesicular transport

Active Chemical Synapse

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Exam I average 81.2% median 84% Scramblase question was not graded Add 1 point to your score