The Effect of Resveratrol on Fat Storage in C. elegans By Chris LoBello

Obesity

Adipocytes

PPARg

Chung and Kurtev- Sirt1 promotes fat mobilization in white adipocytes by repressing PPAR-g. Nature (2004)

Fei and Liu- Relevance of NAC-2, an Na+ coupled citrate transporter, to life span, body size and fat content in Caenorhabditis elegans. Biochemical Society (2004) Wood and Sinclair- Sirtuin activators mimic caloric restriction and delay ageing in metazoans. Nature (2004) b/nature02789_F2.html &blobtype=pdf

Methodology Fluorescent intensity determined by using images taken from microscope to place in Photoshop to determine the strength of the fluorescence Nile Red staining solution (.1microgram/ml) Done up to days 6 to 8 in the protocol Group 4: 100 microliters of resveratrol N= N2 Wild Type Strain Culture Group 3: 50 microliters of resveratrol N= N2 Wild Type Strain Culture Group 2: 10 microliters of resveratrol N= N2 Wild Type Strain Culture Group 1: 1 microliter of resveratrol N= N2 Wild Type Strain Culture Control Group: 0 microliters of resveratrol N= N2 Wild Type Strain Culture Statistical Analysis by ANOVA where p <.05 followed by Scheffe post hoc test The Effect of Resveratrol on Fat Storage in C. elegans

Budget VendorOrder #QuantityDescriptionPriceTotal Price Carolina C. elegans culture kit$73.00 Sigma-AldrichN MG1 100 MG Nile Red Staining $64.20 Sigma-AldrichR MG1100 MG Resveratrol$81.50

Do-Ability C. elegans can be used as a valuable model organism for the study Nile Red Staining will successfully help examine resveratrol’s effect on the C. elegans Results of the staining can be quantified by using Photoshop

Purpose To determine the effects of varying amounts of resveratrol on the fat content of C. elegans

Hypothesis Null Hypothesis: There will be equal amounts of fat content in cells with resveratrol supplemented and without resveratrol supplemented Alternate Hypothesis: There will be less fat content in the cells supplemented with resveratrol, and that there will be an inverse relationship between resveratrol supplementation and fat content

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