THE CHARACTERIZATION OF 4 TIBETAN ARTEMIA STRAINS AND THE HUFA PROFILE DURING THE ENRICHMENT AND STARVATION Xiuling Yu, Naihong Xin, Juju Sun Salt Research Institute

Characterization of 4 Artemia strains 1. Biometrics: 2. Hatching rate 3. Fatty acid profile

4 Tibetan Salt Lakes 4 Tibetan Salt Lakes 1. Gaize lake (GZ) 2. Shuanghu Lake (SH) 3. Nima Lake (NM) 4.Yangnapengco Lake (YP).

Table 1. Location, elevation and surface area of 4 Tibetan lakes Lake Location Elevation (m) Area (km 2 ) GZ 84  05E32  02N YP / / 12 SH 91  10E31  13N NM 86  07E30  28N

Table 2 Biometrics of Artemia from 4 Tibetan lakes

Table 3 Hatching rate of Artemia cysts from 4 Tibetan lakes at 28  C (hrs)

Table 4 HUFA content of decapsulated cysts from 4 Tibetan lakes (mg/g,D.W.)

Enrichment and Starvation Two Artemia strains: 1. Great Salt Lake(EG) 2.Laguoco Lake (AT) Three enrichment diets: 1. DC Selco 2. DC Selco 3. A1 Selco

Conditions: Enrichment:800ml 35ppt seawater, 27  C, density 300/ml(EG), 200/ml(AT), 0.6g/l (two ration) Starvation: 800ml 35ppt seawater, 12  C, Density 900ml/(EG),600/ml(AT), The samples were taken at 6 hrs,12 hrs and 24hrs

Table 5 The HUFA content (mg/g, D.W) of A.franciscana (EG) and A.tibetiana after 24 hours ’ enrichment and during starvation at 12  C.

Note: 24E: after 24 hours enrichment, 6h/12  C : store the enriched nauplii at 12  C for 6 hours, 12h/12  C : store the enriched nauplii at 12  C for12 hours, 24h/12  C : store the enriched nauplii at 12  C for 24 hours

The metabolism of A.franciscana(EG) and A.tibetiana(AT) nauplii enriched with Super Selco during cold storage at 12  C

The metabolism of A.franciscana(EG) and A.tibetiana(AT) nauplii enriched with A1 Selco during cold storage at 12  C

The decrease of DHA/EPA ratio of A.franciscana(EG) and A.tibetiana(AT) nauplii enriched with Super Selco during the cold storage at 12  C%

The decrease of DHA/EPA ratio of A.franciscana(EG) and A.tibetiana(AT) nauplii enriched A1Selco during the cold stored at 12  C