Dormancy of cells and organisms - strategies for survival and preservation NEST - # Acronym: Sleeping Beauty

Partners Esther Lubzens (NIO) Ora Hadas, Assaf Sukenik (KLL) -IOLR – P1 Joan Cerdà, IRTA, Barcelona, Spain – P2 Stefan Hohmann, Goteborg University, Sweden – P3 Melody Clark and Roger Worland, BAS, Cambridge, UK – P4 Richard Reinhardt, Max Planck Institute –Molecular Genetics, Berlin-Dahlem, Germany – P5 Jens Nielson, Danish Technical University, Lyngby, Denmark - P6

Objectives Learning from nature to develop new concepts for cell preservation based on mechanisms with which model organisms tolerate desiccation and/or remain metabolically inactive for long periods

Goals Reveal strategies employed by different organisms that facilitate their long term survival in a dormant form Widening the basis of our knowledge on the molecular details of strategies employed by 5 model organisms Resolve whether these strategies can be used to develop preservation (“ reversible dormancy ”) approaches at ambient temperatures for different cells and organisms * Emphasis on understanding processes that control the establishment and maintenance of dormant stages and the exit from dormancy

Specific goals WP 1 & 2 Discover the genes, proteins and compounds that are associated with the formation of dormant forms and exit from it in: Cyanobacteria Yeast Rotifers WP 3. Discerning the processes associated with desiccation as a survival strategy by global gene expression, proteome and metabolome profiling Arctic springtail killifish embryos

Specific Goals - Continued WP 4. Confirming the role of specific genes in tolerance to desiccation or dormancy by genetic analyses and genetic genetic engineering experiments Yeast Killifish Rotifers (genetically selected strains) WP 5. Discovering common and divergent strategies through synthesis of the genomic, proteomic and metabolomic data of model organisms and of data from other organisms, including plants – in silico models

Specific Goals - Continued WP 6. Publicity to stir and encourage new research direction Scientific publications, scientific meetings Workshop Book Tools: High through approaches; genomics, proteomics, metabolomics and bioinformatics

Partners Ora Hadas, Assaf Sukenik (IOLR)- Cyanobacteria Joan Cerdà, IRTA, Barcelona, Spain - Killifish Stefan Hohmann, Goteborg University, Sweden - yeast Melody Clark & Roger Worland, BAS, UK – Arctic sprintail Richard Reinhardt, Max Planck Institute –Molecular Genetics, Berlin-Dahlem, Germany – Genomic and proteomic platform Jens Nielson, Danish Technical University, Lyngby, Denmark - metabolomic platform

Starting month Leading partner & Participating partners TitleWP Number M0Esther LubzensManagement WP0 M0Esther Lubzens & Ora Hadas- P3, P5, P6 Establishment of dormant stages WP1 M0Stefan Hohmann – P1, P5, P6 Arousal from dormancy WP2 M0Joan Cerdà – P4, P5, P6 Desiccation and revival WP3 M15Joan Cerdà – P1, P3, P5, P6 Engineering /genetic testing WP4 M0Stefan Hohmann & Melody Clark – P1, P2, P5, P6 Common and divergent pathways WP5 M12Esther Lubzens –P2, P3, P4, P5, P6 Dissemination WP6

Pert Chart for SLEEPING BEAUTY