Plant Responses to Signals IV Photomorphogenesis Circadian Rhythms Gravitropism

Signal Transduction general Fig General Signal Transduction Signals, - hormone, - light, - temperature, - gravity, - etc. Receptors, - G-protein linked, - enzyme linked, - protease linked, - etc. Second messengers, - kinase cascades, - calcium concentration, - etc. Responses, - gene expression (+/-), - membrane dynamics, - metabolism - cytoskeleton - etc.

Light is used by plants as a signal, as well as an energy source, –quantity, –quality (wavelength), –direction, –duration. Photomorphogenesis Germination (+/-) Stem length (-) Leaf expansion (+) Flowering (+/-) Phototropism (+/-) Stomatal opening (+) Chloroplast development (+) Pigment synthesis (+) and more...

Action Spectrum, – graph of the magnitude of a biological response to light, –as a function of wavelength. Action Spectra Germination Stem elongation (inhibition)

Molecular Switch …looking for a photoreceptor, Germination hypothesis …look for a photo-reversible pigment. Fig

Absorption vs. Action Spectra …looking for a photoreceptor, Germination

Phytochrome photoreceptor molecule dimer red light FR light Fig

Phytochrome photoreceptor molecule Quantity, Time, Quality. Fig

Phytochrome Location Phtyochrome is a cytosolic protein.

Phytochrome …has multiple functions, Seed Germination, Flowering time (photoperiodism), Entraining (setting) the biological clock, End of day, Stem elongation, Leaf Expansion, Pigment synthesis.

Photoperiodism …flowering times, Long-day plants, …night breaks induce flowering. Short-day plants, …night breaks inhibit flowering. Fig 39.22

Phytochrome …photoperiodism, …use photoreversibility to establish phytochrome function. Fig 39.23

Phytochrome absorbtion spectra, …the wavelengths absorbed by specific pigments. Germination Stem elongation (inhibition) not phytochrome

Cryptochromes blue light photoreceptors (I), …evolved from a light dependent DNA repair enzyme,... across phylogeny, these proteins have been used for many functions, ranging from blue-light- dependent development in plants, blue-light-mediated phase shifting of the circadian clock in insects, to a core circadian clock component in mammals. Stem elongation (inhibition)

Phototropins …mediate phototropism, … contribute to stem, root and leaf movements in response to directional information, to maximize light gathering capacity, and to minimize light damage at high irradiances. Phototropism action spectrum blue light photoreceptors II

Concept Map Phytochrome Action Spectra, Absorption spectra Photoperiodism Photomorphogenesis Cryptochrome Phototropin Photoreversible Functions Signal transduction

Circadian Rhythms Relating to, or exhibiting approximately 24-hour periodicity, –circa around + dies day. Internal Biochemical Oscillators, –found in all eukaryotes, –eubacteria as well. sleep movements Fig 39.21

Circadian Rhythms amplitude Entrainment Circadian processes continue even if light (or dark) is continuous... …amplitude and period entrainment is continuous, - allows fine control. …of response,

~ 480 of 8,000 (tested) genes are under circadian control, ~1,500 (estimated) Arabidopsis genes, or ~6% follow circadian cycles of expression.

Photosynthesis genes... Secondary metabolism (wood, defense).

Gravitropism … the gravity directed growth processes that direct root and shoot orientation during a plants life-cycle, roots, …are positively gravitropic. shoots, …are negatively gravitropic. –about 1.7%, or roughly 500 genes, are transcribed in Arabidopsis when it is re-oriented 90 o.

Gravitropic Set Point 0o0o 90 o 180 o Plant organs orient themselves to the gravity vector.

Starch Statolith Hypothesis Re-orientation of heavy starch grains signals gravity vector. Fig Moss