RhoGTPases and Actin Cytoskeleton Alex André Andres Elena Isabelle Shuoshuo Structural & Functional Predictions for ELMO homologues A Bioinformatic Approach.

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RhoGTPases and Actin Cytoskeleton Alex André Andres Elena Isabelle Shuoshuo Structural & Functional Predictions for ELMO homologues A Bioinformatic Approach

Overview Actin cytoskeleton  Cytoskeletal dynamics in cellular processes  Rho GTPases play role in cytoskeletal remodeling  GEFs and GAPs regulate Rho GTPases  ELMO1 – GEF for Rac

ELMO1 (Engulfment and cell motility protein 1)  CED12 in C.elegans phagocytosis of apoptotic cells and cell motility  Not much known (19 papers in Pubmed)  What shall we do? BIOINFORMATICS

ELMO1 domain organisation

Alignment of the ELMO-domain

Alignment of the PH-domain

Phylogenetic relationship of ELMO1 homologues insects yeasts vertebrates plants Nematodes

Biocomputing and Reality

Prediction of phosphorylation sites in the ELMO1 sequence AKT1 Rac-alpha S/T kinase Hck haematopoetic cell kinase

Structure prediction of PH domain by homology remodelling PH domain identity Geno3D Sequence Identity 23.6% 3 models were predicted by homology modelling

Proper homology modelling can yield significantly more information than conventional analysis

side view overhead view

The ELMO team

“Critiques help us to progress”