Non Homologous End Joining. Homologous Recombination Non Homologous End Joining.

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Presentation transcript:

Non Homologous End Joining

Homologous Recombination Non Homologous End Joining

Differences between HR and NHEJ: Error- free / Error- prone Cell cycle regulated Varies between species HR NHEJ yeastmammals plants

HRNHEJ MHC rearrangement Meiosis Cell-induced Double Strand Brakes

V(D)J recombination

NHEJ Defects SCID (Severe Combined Immune Deficiency). Cancer – genome instability: rearrangements, deletions, amplifications.

Ku 70/80 heterodimer

Ku has a role in telomeres ?

KU 70/80

Ku is critical for telomere maintenance

Ku Plays Several Roles at Telomeres Telomere addition Telomeric silencing

Ku is a multifunctional protein

The Role of NHEJ Proteins in Telomere Biology

Visualization of single double- strand brake

HR NHEJ