Calculate partial pressure of O 2 at Sea level Nitrogen78.08% Oxygen20.95% Water Vapor0 to 4 % Argon0.93% Carbon Dioxide0.036% Neon0.0018% Helium0.0005%

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Calculate partial pressure of O 2 at Sea level Nitrogen78.08% Oxygen20.95% Water Vapor0 to 4 % Argon0.93% Carbon Dioxide0.036% Neon0.0018% Helium0.0005% Methane % Hydrogen % Nitrous Oxide % Ozone % What’s in our air? P gas a = Gas a X P total Gas mix

The respiratory pigments… Why are they important? 100X increase!

O 2 -Hb dissociation curves: Describe activity of Hb at different P O 2 % saturation PO2PO2

O 2 -Hb dissociation curves: P 50 is a measure of O 2 affinity Hb with a high affinity has a lower P 50 Animals that have Hb with high affinity: Hb is saturated when O 2 concentrations are relatively low b/c Hb will not release O 2 unless O 2 levels are very low this kind of Hb favors O 2 uptake (loading) Hb with a low affinity has a higher P 50 Animals that have Hb with low affinity: Hb is only saturated when O 2 concentrations are relatively high b/c Hb is more likely to “let go” of O 2, even if O 2 levels are pretty high this kind of Hb favors O 2 delivery (offloading) HIGH AFFINITY LOW AFFINITY

Comparing dissociation curves… myoglobin P 50 % saturation P O 2 (mmHg) hemoglobin

Weddell Seal Harbor seal Human

Comparing Total O 2 Stores: