20040400 XMUGXQ PFS0601 Principles of Fluorescence Spectroscopy Chemistry Department XMU.

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Presentation transcript:

XMUGXQ PFS0601 Principles of Fluorescence Spectroscopy Chemistry Department XMU

XMUGXQ PFS0601 Chapter Six Methods of Fluorometry

XMUGXQ PFS0601 Methods of fluorometry 6.1 IntroductionIntroduction 6.2 Conventional Fluorometry 6.3 Synchronous Fluorescence ScanSynchronous Fluorescence Scan 6.4 3D fluorescence spectroscopy3D fluorescence spectroscopy 6.5 Derivative fluorescence spectroscopyDerivative fluorescence spectroscopy

XMUGXQ PFS Introduction Models Steady-state fluorescence measurement Time-domain fluorescence measurement Time resolved fluorescence Frequency-domain fluorescence measurement I0I0 t I0I0 t I0I0 t Light source

XMUGXQ PFS Synchronous Fluorescence Scan SFS Conventional Fluorometry Excitation spectrum Emission spectra F F em Scan ex at a certain em Scan em at a certain ex

XMUGXQ PFS0601 Synchronous fluorescence scan SFS models Scan ex and em simultaneously Constant-wavelength SFS Constant-energy SFS Variable-angle SFS

XMUGXQ PFS0601 Constant-wavelength SFS

XMUGXQ PFS0601 Narrowing spectral band & simplifying emission spectrum

XMUGXQ PFS0601 Narrowing spectral band & simplifying emission spectrum

XMUGXQ PFS0601 Multi-component analysis F F em CFS F em SFS

XMUGXQ PFS0601 Theory Where Ex is the excitation wave function at a given wavelength; Em is the emission wave function at a given wavelength; l is the thickness of the sample; K is the instrumental geometry and related parameters.

XMUGXQ PFS0601 Choosing the  Generally, the stoke’s shift F

XMUGXQ PFS0601 Choosing the 

XMUGXQ PFS0601 Choosing the 

XMUGXQ PFS0601 Constant-energy SFS PAHs

XMUGXQ PFS0601 Variable-angle SFS

XMUGXQ PFS0601 Variable-angle SFS

XMUGXQ PFS0601 Variable-angle SFS

XMUGXQ PFS0601 Variable-angle SFS

XMUGXQ PFS0601 Variable-angle SFS

XMUGXQ PFS0601 Variable-angle SFS

XMUGXQ PFS0601 Reference Trends in analytical chemistry, 21(12): , 2002

XMUGXQ PFS dimensional fluorescence spectroscopy

XMUGXQ PFS0601 Contour 3D spectra

XMUGXQ PFS0601 Finger printing

XMUGXQ PFS0601 Volume integral

XMUGXQ PFS0601 Derivative fluorescence 芘

XMUGXQ PFS0601 Derivative fluorescence 峰距法切线法 峰零法

XMUGXQ PFS0601 application b a

XMUGXQ PFS0601 application

XMUGXQ PFS0601 application

XMUGXQ PFS0601 application Finger printing

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