A Biology and Engineering Cooperative Project James W. Stevens M. Karen Newell University of Colorado at Colorado Springs 30 April 2005
general background connection between cellular metabolic pathways and immune system response surface signals tied to mitochondrial activity and fuel
cooperative effort biology –science driver –cell cultures growing conditions –cell counting and physical preparation engineering –data acquisition –temperature measurements –data reduction –data smoothing
background-this project 2 mouse leukemia lines L1210 – not drug resistant L1210/ddp– drug resistant cultured to 10^6 to 10^8 cells stressed with bleach –measurable, repeatable differences –different culture conditions, metabolic states
data analysis first law of thermodynamics:
data smoothing least square curve fit: minimize
summary leverage complementary capacities– specific to people and project involve students plunge into a new area
other cooperative efforts at UCCS biomechanics –biology, mechanical engineering orthopedic mechanics –MERIC, biology, mechanical engineering feedback controls –MAE, biology microgravity – MAE assistive technologies – MATH, biology