iGEM2009 SHORT COURSE Lesson1 Introduction to iGEM and Synthetic Biology

什麼是合成生物學 ?? Synthetic biology is an emerging area of research that can broadly be described as - the design and construction of novel artificial biological pathways, organisms or devices, or -the redesign of existing natural biological systems. YM-Bioinfo What is Synthetic Biology?

1. 傳統的生物學 Reverse engineering: Taking apart sequenced whole genomes through cross-genome comparison to see how they work. 2. 合成生物學 Forward engineering: Design and build engineered biological systems to test biological hypothesis. Engineering approaches to the study of biological sciences

A Robot Building Kit

Systems Parts & Fabrication Design Tools Registries Synthesis Measurement Applications The Goal

8 什麼是 iGEM??  It is an abbreviation of the "international Genetically Engineered Machine" competition.  It is organized by Massachusetts Institute of Technology  It is a Synthetic Biology competition of students.  The objective of iGEM competition is to imagine, engineer, and assemble an engineered biological system using DNA parts.  the completed project will then be presented at iGEM jamboree in Boston in November.

Timeline 2003 MIT class 2004 MIT class 2004 Synthetic Biology Competition (5 teams, 1 country) iGEM 2005 (13 teams, 4 countries) iGEM 2006 (37 teams, 15 countries) iGEM 2008 (85 teams registered, 24 countries) iGEM 2007 (54 teams, 19 countries) We are Here!!

BioBrick is a standard for inter-changable parts, developed to build biological systems in living cell Biobrick

Standard Vector

Biobrick ligation

Biobricks for Synthetic Biology YM-Bioinfo

Promoter RBS (Ribosome Binding Sites) Protein coding sequence Protein tags and modifiers Reporter Inverter Terminator Primer

Single promoter, single gene Single promoter, single reporter Single promoter with fusion protein Multi-promoter, multi-gene

Wiki A wiki is a web application that allows users to add content, as on an Internet forum, but also allows anyone to edit the content. Wiki also refers to the collaborative software used to create such a website

Feature of Wiki Allow users to free create and edit web page content using any web browser Allow a user to add content, like Internet forum, but the content to be edited by any other user Supports hyperlinks Can create new pages and crosslink between internal pages

IGEM 官方 WIKI 2008 公開 WIKI 2008 內部 WIKI(CRAZY IDEA)

Brainstorming Focus on quantity No criticism Unusual ideas are welcome Combine and improve ideas

Structure of research proposal Motivation Goal and specific aims Introduction Materials and methods Anticipated results Discussions Conclusions