BRCA2 Blue: Rad51; Green: BRCA2

Statistics 1/8 women will get breast cancer 80% have no family history of cancer 20% has a family member with some germline history of cancer 50% are caused by a mutation in either BRCA1 or BRCA2 Notes: - Risk developing cancer averages about 13% with a BRCA2 mutation about 60-80% - Risk of developing ovarian cancer 1.5% with a BRCA2 mutation about 15-27% -About 70 to 80% of all familial ovarian cancers are due to mutant germline alleles of BRCA1 and 2

What process is BRCA2 involved? BRCA2 and Rad51 increased levels of expression During S phase of cell cycle Induce mutations in Drosophila to see what process BRCA2 is involved in. homepage.mac.com/enognog/cell%20cycle.jpg

Homologous Recombination Sung, P. et al. J. Biol. Chem. 2003;278:

Rad51 Sung, P. et al. J. Biol. Chem. 2003;278:

Rad51 Nature Genetics 27, (2001)

BRCA2 Narod, Nature Reviews: Cancer (2004)

BRCA2 Powell, Oncogene (2003)

BRCA2 CREDIT: H. YANG ET AL., SCIENCE 297, 1837 (13 SEPTEMBER 2002)

Tumor Suppressor

Knockout Mice Knockout BRCA2 genes give a lethal phenotype. R. Weinberg, Cancer Biology BRCA2 requires 2 mutant copies to have the ability for the mouse to develop a tumor.

How BRCA2 Causes Cancer Nature Genetics 27, (2001)