T h e U n i v e r s i t y o f S o u t h e r n Q u e e n s l a n d Rob Learmonth Fluorescence fables for the workshop of FABLS www.usq.edu.au/biophysci.

Slides:

Advertisements

Similar presentations

Day 3 Platforms Biological sensors Chemical sensors.

Advertisements

Absorption of photon elevates chromophore to excited state.

Lessons Learned from Measuring Cell Response by Quantitative Automated Microscopy FDA Workshop, Potency Measurements for Cellular and Gene Therapy Products,

Fabian Lema *I’m not set on the title of the project W1 Remote Monitoring of Android Devices* University Of Queensland, Australia.

Department of Cell and Molecular Biology. Recent Research Projects 1) Conformational dynamics of ribosomal proteins: both prokaryotic and eukaryotic “stalk”

Contact: Annika Enejder, Technical BioImaging TIF045 5p 24:th October-9:th December To see is to understand...

Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display. 1 The Study of Microbial Structure: Microscopy and Specimen.

Laboratory Tools in Microbiology

Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings 1 Chapter 3 Observing Microorganisms Through a Microscope.

MB304 Industrial & Environmental Biotechnology Module 1 Industrial Microbiology 1 Prepared by: Ms. Lim Yin Sze.

Breaking the Diffraction Barrier: Super-Resolution Imaging of Cells Edgar Ferrer-Lorenzo, Nicole Gagnon, Anna Torre 1.

Comparison and Implications of Three Optical Microscopy Data Acquisition Modalities James Butler Ph.D. Nikon Instruments, Inc. Widefield Fluorescence Confocal.

Microbiology: What is a microbe?

Rapid identification of microorganisms using optical spectroscopy  Spectroscopic characterisation of yeast strains and lactic acid bacteria, distinction.

Chemical Structure of the Chromophore Biosynthesis of the Chromophore Critcial dehyrogenation reaction to juxtapose aromatic group with imidazlinone.

Helena Nevalainen Peter Bergquist Junior Te’o Ewa Goldys Shoba Ranganathan Marko Nykänen Debra Birch Hong Yu How to retool cellular factories A systems.

Fluorescence and fluorescence- lifetime imaging microscopy (FLIM) to characterize yeast strains by autofluorescence H. Bhatta a, E.M. Goldys a and J. Ma.

Lecture 3/4 Rob Phillips California Institute of Technology (Block et al.) (Wuite et al.)

Immunofluorescence Microscopy. Immunofluorescence Microscopy: When an antibody, or the antiimmunoglobulin antibody used to detect the antibody is labeled.

Quantitative Techniques CE 742: Advanced Topics in Environmental Engineering.

Objectives: Ch. 7 and 18 Distinguish between the concepts of decontamination and sterilization, “cidel” vs “static”. Identify the common heat method practices.

Biomedical Imaging and Sensing Andrei Zvyagin Centre for Biophotonics and Laser Science Physics, School of Physical Sciences Biomedical Engineering, School.

The MOSS Camera on H-1NF Clive Michael John Howard PRL,RSPhysSE,ANU Outline: MOSS (Modulated Optical Solid-State Spectrometer) Camera imaging Results and.

FSN 1500 Week 7, 8 The Cell Introduction The study of biological cells and the potential applications (e.g., stem cell research, cloning, gene therapy)

HAB Case Studies Current Automated Capabilities & Future Needs Automated submersible flow cytometry Flexible targets, ecological context (MVCO examples)

Evaluating Potential Drug Therapies Mike Shuler Biomedical Engineering.

David Sampson School of Electrical, Electronic, & Computer Engineering.

Using Nanotechnology in Medical Imaging and Diagnosis Alisha Shutler Introduction to Nanotechnology December 1, 2007.

INTERREG IV A « 2 Mers Sea Zeeën » The DYMAPHY project is part of the European cross- border cooperation programme INTERREG IV A « 2 Seas ». The project.

What are the problems? Lower yield potential of varieties

Advanced Higher Mechanics Linear and Parabolic Motion Mathematical Techniques for Mechanics Force, Energy and Periodic Motion AH.

A Raman spectrum gives a unique chemical signature of a specimen The Raman microscopy is used extensively in the following applications: Pharmaceuticals.

Imaging research and consultancy available at the University of Ulster  Over 30 years experience  Expert assistance from factory trained staff  Reference.

Nanomechanics Track Summary NNI Instrumentation and Metrology Grand Challenge Workshop January 29, 2004.

Role of Glass Composition in Waveguide Fabrication with Femtosecond Laser Pulses Denise Krol, University of California, Davis, DMR Tightly focused.

Microscopes. Micrographia Robert HookeRobert Hooke First report of cell structureFirst report of cell structure corkcork First illustrated book.

Confocal Laser Scanning Microscopy: general considerations and techniques Simone Bossi.

Research interests of Dr Konstantinos Papadimitriou Research associate Laboratory of Dairy Research Department of Food Science and Human Nutrition Agricultural.

SCM 330 Ocean Discovery through Technology Area F GE.

MEMBRANE FILTRATION All images included in this file are reproduced with permission from the copyright holders and/or Companies producing the respective.

Over 28 Years of Machine Tool Monitoring Expertise.

SCM 330 Ocean Discovery through Technology Area F GE.

Objectives: Ch. 9 and 10 Distinguish between the concepts of decontamination and sterilization, “cidel” vs “static”. Identify the common heat method practices.

Imaging Poliovirus Entry in Live Cells Brandenburg et al PLoS Biology 2007 Purpose: To chacterize early stages of poliovirus infection in living cells.

0-10V Ballast Dimmer HDL-MRDA HDL-MRDA channel 10A DC0 － 10V output module is a multifunction control module. It has 6 channel relay.

Freezing phase scheme for complete-field characterization and coherent control of femtosecond optical pulses Jung Y. Huang, Ci L. Pan, and J. I. Chyi An.

Date of download: 5/28/2016 Copyright © 2016 SPIE. All rights reserved. (a) A stack of representative three-dimensional images of 80-nm AuNPs embedded.

1 Modelling and Simulation EMBL – Beyond Molecular Biology Physics Computational Biology Chemistry Medicine.

Chapter 4, 5, 6. Chapter 4: Microscopy, Staining, and Classification.

Do Now Turn to your notes packet. Continue working on the page of questions we left off on (#14, 15, and 16). Go to next page and do #17.

Topic 1 Microscopes and Microscopy. Light Microscopes  How do they work?  Optical magnification  Images pass through a lens or a series of lenses 

Observing Microorganisms Through a Microscope

Advanced Higher Mechanics

Respiration 2012;83:74–80 - DOI: /

Microbiology: A Systems Approach

BRC Science Highlight Mechanism of Ionic Liquid Toxicity and Rational Engineering of a Tolerant Yeast Strain Objective To identify genetic determinants.

Background With new accelerators delivering beams always smaller and more energetic, requirements for very precise beam alignment become more and more.

Burton's Microbiology for the Health Sciences Chapter 2

Deep brain single optical fiber fluorescence imaging

Flow cytometry of wild‐type and ΔseqA cells.

Observing Microorganisms Through a Microscope

أنماط الإدارة المدرسية وتفويض السلطة الدكتور أشرف الصايغ

The Study of Microbial Structure: Microscopy and Specimen Preparation

Presentation to: NIST Metrology Workshop Metrology for Atomic Precision Fabrication John Randall Chief Technical Officer Zyvex Corp.

Scalar theory of diffraction

Experimental setup.A, topology models of the overexpressed membrane protein GFP fusions. Experimental setup.A, topology models of the overexpressed membrane.

Observing Microorganisms Through a Microscope

Exosome-mediated inhibition of T cells is reversible.

Bioprocess Technology. Upstream bioprocessing process from early cell isolation and cultivation, first step in which microbes/cells are grown,

Presentation transcript:

T h e U n i v e r s i t y o f S o u t h e r n Q u e e n s l a n d Rob Learmonth Fluorescence fables for the workshop of FABLS

T h e U n i v e r s i t y o f S o u t h e r n Q u e e n s l a n d Rob Learmonth: Interests in fluorescence Broad areas in fluorescence: Education & ResearchEducation & Research spectroscopyspectroscopy development & application of new techniques utilising CLSM & MPLSMdevelopment & application of new techniques utilising CLSM & MPLSM To study: cell membrane structure & dynamicscell membrane structure & dynamics spectroscopic signatures of microbesspectroscopic signatures of microbes –With Ewa Goldys, Hemant Bhatta microbial adaptation processes & mechanismsmicrobial adaptation processes & mechanisms microbial responses to membrane-active agentsmicrobial responses to membrane-active agents brewing & wine fermentationbrewing & wine fermentation fluidity modulation in transgenic frost tolerant wheatfluidity modulation in transgenic frost tolerant wheat

T h e U n i v e r s i t y o f S o u t h e r n Q u e e n s l a n d Local developments in instrumentation Centre for Advanced Light Microscopy UQ BrisbaneCentre for Advanced Light Microscopy UQ Brisbane –2001 RIEF UQ/USQ: CLSM & MPLSM –2003 LIEF UQ/USQ: CLSM & MPLSM (2) Macquarie UniversityMacquarie University –2003 LIEF MU/ US/ UNSW/ UTS/ UN/ UW / USQ: Multifunctional CLSM / MPLSM/ FLIM USQUSQ –SLM/Aminco Bowman analog fluorometer –ISS photon-counting fluorometer –BIORAD MRC600 CLSM

T h e U n i v e r s i t y o f S o u t h e r n Q u e e n s l a n d Current and emerging challenges in fluorescence For monitoring cell status during bioprocesses development ofFor monitoring cell status during bioprocesses development of –rapid techniques –simple instrumentation Flow cytometry of microorganismsFlow cytometry of microorganisms Development of new specific probesDevelopment of new specific probes Improvement of resolution in optical microscopyImprovement of resolution in optical microscopy Development of live-cell FISH?Development of live-cell FISH?