20x UV 20x Visible Relative Intensity Pixels Relative Intensity Field Illumination - 20x Objective.

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Presentation transcript:

20x UV 20x Visible Relative Intensity Pixels Relative Intensity Field Illumination - 20x Objective

UV Field Illumination 100X Objective (1.4 NA) 12.5% intervals INTENSITY=

20x Vis 10x Vis (2/4/99) 10x UV 20x UV Good Poor Field Illumination

5x10x20x40x63x 5x10x20x40x63x Visible Ultraviolet Field Illumination

Axial Resolution - Lens Comparison 40x63x100x 610nm 390nm 280nm Axial Distance (  m) FWHM

190 nm 410 nm

647nm 488nm568nm

Focal Check Beads

Comparison of PMT Values

Intensity Axial Profile Mirror - VisibleBeads - UV/VisibleFocal Check Beads Spectral Registration Check Methods

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