Biochemistry Ch6. Exploring Gens 阮雪芬 NTUT Oct 14, 2002

Restriction Enzyme

Specificities of Some Restriction Endonuclease

Gel Electrophoresis

Southern Blotting

Strategy of the Chain-termination Method for Sequencing DNA

Fluorescence Detection

A Complete Genome

Solid-phase Synthesis of a DNA Chain

The First Cycle in PCR

Multiple Cycles of PCR (I)

Multiple Cycles of PCR (II)

Multiple Cycles of PCR (III)

DNA and Forensics

Joining of DNA Molecules by the Cohesive-end Method

Formation of Cohesive DNA

Choices Vectors for DNA Cloning in Bacteria pBR322 plasmid Lambda phage M13 phage

Genetic Map of The Plasmid pBR322

Aternative Infection Modes for Lambda Phage

Mutant Lambda Phage as a Cloning Vector

EM of M13 Filaments Phage

M13 Phage DNA a Cloning and Sequencing Vector

Creation of a Genomic Library

Screening a Genomic Library for a Specific Gene

Probes Generated From a Protein Sequence

Diagram of a Yeast Artificial Chromosome (YAC)

Chromosome Walking

Synthesis of Proinsulin by Bacteria

Formation of a cDNA duplex

Screening of cDNA

Gene Expression Analysis Using Microarrays

Monitoring Changes in Yeast Gene Expression

Microinjection of DNA

Transgenic Mice

Rat Growth Hormone- metallothionein Gene Construction

Gene Disruption by Homologous Recombination

Consequences of Gene Disruption

Tumors in Plants

Ti Plasmids

Electroporation

Oligonucleotide-directed Mutagenesis

Cassette Mutagenesis

The Techniques of Protein Chemistry and Nucleic Acid Chemistry are mutually Reinforcing