Prion Project - Update Mass Spectrometry of Prions Andy Lo, Béla Reiz, Messele Fentabil Dr. Liang Li Department of Chemistry University of Alberta.

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Presentation transcript:

Prion Project - Update Mass Spectrometry of Prions Andy Lo, Béla Reiz, Messele Fentabil Dr. Liang Li Department of Chemistry University of Alberta

Mass Spectrometry (MS)  Mass Spectrometry is an instrumental method used to identify chemical substances by means of the separation of their gaseous ions using magnetic and/or electric fields according to their mass-to-charge ratio.

MS in PROTEOMICS  Molecular weight of proteins  Masses of peptides in a mixture  Masses of fragment ions of a peptide

Experimental Procedure Database Search

Protein MS

Experimental Procedure Database Search

PrP Tryptic Digest - MS

PrP&Myo Tryptic Digest - MS

Experimental Procedure Database Search

PrP&Myo Tryptic Digest LC-MALDI-MS

Experimental Procedure Database Search

PrP&Myo Tryptic Digest LC-MALDI-MSMS

Experimental Procedure Database Search

Database Search Results

PrP Chymotryptic Digest LC-MALDI

PrP Chymotryptic Digest Results

Future Work Chemical surface modification of surface exposed residues using:  N-bromosuccinimide (NBS) for Trp exposed residues  Hydroxylation of surface exposed Cys, Trp, Tyr, Met

Thanks!  Dr. Liang Li  Andy and Messele  All Li Group members

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