The miracle of N 2 ase: How does it do it? N2 bond energy = 945 kJ/mol!!!! :NN: N2 + 8H+ + 8 e- + 16 MgATP  2 NH3 + H2 + 16 MgADP + 16 Pi.

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Presentation transcript:

The miracle of N 2 ase: How does it do it? N2 bond energy = 945 kJ/mol!!!! :NN: N2 + 8H+ + 8 e MgATP  2 NH3 + H MgADP + 16 Pi

Root nodules formed by Rhizobium bacteria living symbiotically through nitrogen fixation.

N2 + 8H+ + 8 e MgATP  2 NH3 + H MgADP + 16 Pi

EPR used to monitor S= 3/2 signal under a variety of conditions FeMo protein (FeMoco site)Fe protein

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P-cluster Reduced stateOxidized state Strange Sulfur: CN= 6: Creates flexibility for Fe’s?

Nitrogen Binding to the FeMo cofactor of Nitrogenase, Johannes Schimpl, Helena M. Petrilli, and P.E. Blöchl, J. Am. Chem. Soc. 125, (2003), TU Clausthal Institute of Theoretical Physics Department of Applied Theoretical Physics