G.007 Hardware open-CAD 30 msec move/settle 42 kbp/sec Gbp/run

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G.007 Hardware open-CAD 30 msec move/settle 42 kbp/sec 10-60 Gbp/run Xenon Light EM-CCD Camera 30 msec move/settle 42 kbp/sec 10-60 Gbp/run $124,000 Fluidics Autofocus Objective Stage 3U Imaging 2U Sequencing

Hardware: Polonator G.007 DIY PersonalGenome.org Nikon, Hamamatsu, Alcott, Prior, Sutter, Bioptechs, PI, Uniblitz, Superlogics Shendure, Porreca et al. 2005 Danaher, Hamamatsu, Leica $124K with computer & 1 yr service Contact: kevin.mccarthy@danahermotion.com Rich Terry et al. 2007 DIY PersonalGenome.org

36 Flowcells G.007 60M beads per flowcell Filling with beads PersonalGenome.org

Higher density & accuracy Gel array, 12M beads Shendure, Porreca et al. 2005 Glass array, 60M beads Kim, Porreca et al. 2007 1.6M reads 12M reads (M. Umbarger)