DNA Nucleosides Deoxyadenosine (A) Deoxyguanosine (G) Deoxycytidine (C) Deoxythymidine (T)

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Presentation transcript:

DNA Nucleosides Deoxyadenosine (A) Deoxyguanosine (G) Deoxycytidine (C) Deoxythymidine (T)

Synthetic Objective G - A - T - T - A - C - A C -phosphate-A -phosphate-T -phosphate- A -phosphate-G- phosphate-A The problem is like the problem of protein synthesis Protection ! The solution?

Phosphoramidites A G C T

DMT protection A G C T

DMT deprotection

Protect amines

Phosphoramidite converted to Phosphite A C Silica surface tetrazole - diisopropylamine

Remove the dimethoxyltrityl group with acid CHCl 2 COOH

Next step tetrazole