Biomedical Imaging and Sensing Andrei Zvyagin Centre for Biophotonics and Laser Science Physics, School of Physical Sciences Biomedical Engineering, School.

Slides:



Advertisements
Similar presentations
Hyperspectral two-photon near- infrared cancer imaging at depth Nikolay S. Makarov, Jean Starkey, Mikhail Drobizhev, Aleksander Rebane, Montana State University,
Advertisements

Fluorophores bound to the specimen surface and those in the surrounding medium exist in an equilibrium state. When these molecules are excited and detected.
R. Hui Photonics for bio-imaging and bio- sensing Rongqing Hui Dept. Electrical Engineering & Computer Science, The University of Kansas, Lawrence Kansas.
OUTLINE Introduction „Tera-to-Nano“: Our Novel Near-Field Antenna 80 GHz CW Frequency Domain Measurements Picosecond Pulse Time Domain Measurements 2D.
Lecture 3 Kinetics of electronically excited states
Optical Tweezers F scatt F grad 1. Velocity autocorrelation function from the Langevin model kinetic property property of equilibrium fluctuations For.
Optical Coherence Tomography Zhongping Chen, Ph.D. Optical imaging in turbid media Coherence and interferometry Optical coherence.
Measuring Fluid Properties on a Microscopic Scale Using Optically Trapped Microprobes Mark Cronin-Golomb Biomedical Engineering Tufts University.
1 Swept Source Frequency Domain Optical Coherence Tomography Swept Source Frequency Domain Optical Coherence Tomography Anurag Gupta University of Rochester,
Lasers, molecular physics, optics, applications and all that … Brian J. Orr (CLA, Macquarie University)
LASERS - PARTICLE SIZE ANALYSIS
Optical Coherence Tomography
Multipurpose analysis: soil, plant tissue, wood, fruits, oils. Benchtop, portable Validation in-built, ISO compliant Little or no sample preparation. Rapid.
Time out—states and transitions Spectroscopy—transitions between energy states of a molecule excited by absorption or emission of a photon h =  E = E.
Student “#2” OPTICAL SCAN RELAY IN CONFOCAL LASER SCANNING MICROSCOPY.
Common types of spectroscopy
Photo-Thermal Coherent Confocal Microscope This work is supported in part by the Center for Subsurface Sensing and Imaging Systems, under the Engineering.
Biomedical optics brought to you by the BIOP center A joint initiative between the Technical University of Denmark, Risø National Laboratory and.
Boston University MRI – Development of a Holographic Nanoscale Optics Facility Development of a Holographic Nanoscale Optical Facility  Sub-20nm optical.
INTRODUCTION TO ANALYTICAL CHEMISTRY ERT 207 ANALYTICAL CHEMISTRY SEMESTER 1, ACADEMIC SESSION 2015/16.
The isosurface is a 3D reconstruction of the DiO dataset. The surface structure exhibits the shape of the dendritic spine and color exhibits the concentration.
David Sampson School of Electrical, Electronic, & Computer Engineering.
ECE 299 Holography and Coherent Imaging Lecture 1 Gabor Holography David J. Brady Duke University Lecture 1: Gabor Holographywww.disp.duke.edu/~dbrady/courses/holography.
Lineshape and Sensitivity of Spectroscopic Signals of N 2 + in a Positive Column Collected Using NICE-OHVMS Michael Porambo, Andrew Mills, Brian Siller,
Ultrafast carrier dynamics Optical Pump - THz Probe Ultrafast carrier dynamics in Br + -bombarded semiconductors investigated by Optical Pump - THz Probe.
Terahertz Applications by THz Time Domain Spectroscopy
Sowmya Vasa, Umar Alqasemi, Aditya Bhargava. Objectives This paper aims in bringing out a novel light microscopy method called Focal Modulation Microscopy.
Project #3: Optical Method of Detection and Analysis of Biological Molecules Faculty Mentor: Dr. Anastasios Angelopoulos Graduate Student Mentor: Mr. Srivathsan.
Summarized by: Name: AGNES Purwidyantri Student ID No: D
Photothermal Therapy Nicholas Ellens MBP September 2010.
Ferroelectric Nanolithography Extended to Flexible Substrates Dawn A. Bonnell, University of Pennsylvania, DMR Recent advances in materials synthesis.
1 Life Analytical Chemistry-Molecular Imaging (MI): Optical Imaging Gaolin Liang (梁高林), Ph. D. Professor, Ph. D. Advisor Deptartment of Chemistry University.
Biophotonics and medical imaging
  Examining how much light is absorbed by a compound’s sample at various wavelengths  Spectrum peaks—  Indicates the wavelengths associated with electrons’
M. J. Levene, W.W. Webb et al. Science (2003).
Dr. Yingtian Pan’s Lab Unjoo Lee. About him  He is an associate Professor
CONSTRUCTION OF A RAMAN TWEEZER SYSTEM FOR INVESTIGATION OF BIOLOGICAL MOLECULES Günay Başar İstanbul Teknik Üniversitesi International.
BMI2 SS07 – Class 5 “OCT” Slide 1 Biomedical Imaging 2 Class 5 – Optical Coherence Tomography (OCT) 02/20/07.
Using Technology to Study Cellular and Molecular Biology.
Dimethyl sulfoxide (DMSO) diffusion in skin tissue Marina D. Kozintseva, Alexey N. Bashkatov, Elina A. Genina, Valery V. Tuchin Department of Optics and.
Photoacoustic Tomography The Future Of Medical Imaging Techniques
By David Tse Mentor: Andreas H. Hielscher, Ph.D Columbia University Biomedical Engineering Department 500 West 120th Street ET351 Mudd Bldg., MC8904 New.
Date of download: 6/21/2016 Copyright © 2016 SPIE. All rights reserved. Scanning mechanisms for (a) conventional FD-OCT and (b) streak-mode FD-OCT. Figure.
Date of download: 6/21/2016 Copyright © 2016 SPIE. All rights reserved. The schematic of the spectral domain OCT imaging system used in this study. Image.
Date of download: 6/22/2016 Copyright © 2016 SPIE. All rights reserved. Schematic of spectral-domain optical coherence tomography (SD-OCT) system and forward-facing.
Biophotonics and Photonic Crystal Fibers (PCF)
Phase-Resolved Optical Frequency Domain Imaging Of The Human Retina On The Reliable Discrimination Of Retinal Blood Flow Master of Physics Symposium Leah.
Literature Survey of Spectroscopic OCT Tuan-Shu Ho 1.
UV/VIS SPECTROSCOPY.
Graduate Institue of Electro-Optical Engineering Course Map
ANALYTICAL CHEMISTRY deals with methods for determining the chemical composition of samples. Dr Seemal Jelani ENVR-303 6/16/2018.
EXPERIMENTAL TECHNIQUES Steady-state techniques In radiolytic investigations the conversion (i.e., the ratio of transformed and starting molecules) is.
Optical Coherence Tomography
Photo acoustic tomography
OPTICAL MONITORING OF PHOTOSENSITIZER DIFFUSION INTO TISSUE
Simulating super-resolution structured illumination microscopy
Introduction to Biosensors
Marina D. Kozintseva1, Vyacheslav I. Kochubey1, Alexey N
COLOR MAPPING OF ONE SPECIFIC VELOCITY OF A FLOW WITH COMPLEX GEOMETRY USING OPTICAL COHERENCE TOMOGRAPHY Potlov A.Yu, Frolov S.V., Proskurin S.G. Biomedical.
Figure 1 NIR fluorescence is more suitable for
Complex Nanophotonics
NEUROIMAGING TECHNIQUE USING TIME-RESOLVED DIFFUSE OPTICAL TOMOGRAPHY AND INHOMOGENEITY LOCALIZATION ALGORITHM Potlov A.Yu, Frolov S.V., Proskurin S.G.
Science, 2010, 330, Room-Temperature Detection of a Single Molecule’s Absorption by Photothermal Contrast A. Gaiduk, M. Yorulmaz, P. V. Ruijgrok,
Colourimetry The absorption of light by a coloured solution is directly related to the concentration of the solution. Colourimetry determines the concentration.
2 types of passive transport
ECE 299 Holography and Coherent Imaging
OCT (Optical Coherence Tomography) Time domain Frequency domain Coherence length.
from W. Demtröder “Molecular Physics”
Imaging techniques for next generation plant cell biology.
Photothermal spectroscopy Rotem Neeman & Yonat Milstein
Presentation transcript:

Biomedical Imaging and Sensing Andrei Zvyagin Centre for Biophotonics and Laser Science Physics, School of Physical Sciences Biomedical Engineering, School of Information Technology and Electrical Engineering The University of Queensland

Projects Coherence-domain imaging techniques, as applied to cells and live biological tissue  Full-field Fourier domain optical coherence tomography (3F-OCT)  Synthetic-aperture confocal microscopy Dialysis-assisted fibre optic spectroscopy for drug sensing in situ

Full-field Fourier domain OCT 3D-data cube is acquired Contains comprehensive information on the sample 3D structure and its optical scatter Signal-to-noise ratio is optimized Suitable for ophthalmology applications in vivo Relies on rapid broadly-tuneable laser swept-source

Dialysis-assisted fibre optic spectroscopy Fluorophores Tissue constituents Illumination To spectrum analyzer Setup for measuring fluorophore concentration in tissue using DAFOS. Inset, DAFOS sampling head. Illumination light guided through a fibre F, is emerging from the dialysis membrane DM, tip. Illumination fibre Microdialysis membrane Tissue Collection fibre Needle ibreibre F DM Small molecules, e.g. drugs, enter the sampling head and detected spectroscopically Compared with traditional microdialysis tool, DAFOS is rapid The equilibrium between the solute and intercellular fluid is established via diffusion, DAFOS is potentially sensitive and reliable The use of high-fluence UV radiation is now possible, as DAFOS can be light- tight, and prevents large protein complexes from entering the sampling head Enables in vivo applications of a range of spectroscopic techniques

DAFOS Time response of DAFOS signal Setup for measuring fluorophore concentration in tissue using DAFOS. Inset, DAFOS sampling head. Illumination light guided through a fibre F, is emerging from the dialysis membrane DM, tip.

The preliminary numerical simulations considering existing tapering technology. The ultimate sub-nM sensitivity, Lize et al., Opt Expr 12 (2004) Tong et al., Nature 426 (2003) Centre for Ultra-high Bandwidth Devices for Optical Systems (CUDOS) Absorption DAFOS