What is SV40 T-antigen? What does it do? Presented by 李柏毅 Genome Engineering Laboratory, Institute of Bioinformatics, National Yang-Ming University 2003/12/12.

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What is SV40 T-antigen? What does it do? Presented by 李柏毅 Genome Engineering Laboratory, Institute of Bioinformatics, National Yang-Ming University 2003/12/12

2 Simian Virus 40 Hosts: monkeys and mice DNA virus with 5,227 base pairs Origin Early mRNAs Late mRNAs 5’ SV40

3 SV40 T-antigen AUG UUA Early pre-mRNA Large T mRNA Small T mRNA AAA n Large T 94kd protein Small T 17kd protein

4 Large T-antigen Delete a.a. Delete 136-C terminus Addition a.a to β-galactosidase and pyruvate kinase Delete a.a. Immunofluorescence microscopy Daniel Kalderon, Bruce L. Roberts, Alan E. Smith, A Short Amino Acid Sequence Able to Specify Nuclear Location, Cell 39: , 1984

5 Tumor Virus Human cells are semipermissive cell

6 Tumor Virus SV40 Large T Ad5 E1A 13S Ad5 E1A 12S Ad5 E1B 55K Rb-bindingP53-binding Release transcription factor for Rb Protein binding sites

7 THANKS!

8 Hahn WC, et al,. Creation of human tumor cells with defined genetic elements. Nature 400, 464–468 (1999)