Quantification des structures 3D à partir de coupes histologiques sériées R. Kraftsik.

Slides:



Advertisements
Similar presentations
Adjusting a Microscope 1Center components on optic axis 2Focus objective 3Focus condenser 4Adjust illumination lamp voltage (intensity) iris diaphragm.
Advertisements

Neuro-Imaging High Resolution Ex-Vivo MRI Ex-Vivo DTI of Brain Stem
Morphometric or 3D reconstruction Chinmaya Sadangi.
Imaging early embryos and stem cells Centre for Trophoblast Research Anne McLaren Laboratory for Regenerative Medicine University of Cambridge Kathy Niakan.
Introduction to Computers Section 10A. home Presentation Programs Provide powerful design tools to outline, create, edit, arrange and display complex.
Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display. 1 The Dark-Field Microscope Image is formed by light reflected.
Image Analysis: To utilize the information contained in the digital image data matrix for the purpose of quantification. 1)Particle Counts 2)Area measurements.
What is vision Aristotle - vision is knowing what is where by looking.
Deconvolution of Laser Scanning Confocal Microscope Volumes: Empirical determination of the point spread function Eyal Bar-Kochba ENGN2500: Medical Imaging.
MSU/CSE Fall Computer Vision: Imaging Devices Summary of several common imaging devices covered ( Chapter 2 of Shapiro and Stockman)
Axonal morphometry n Maxsim n NeuronTracer n Divers Real axons (& dendrites) Parameters & Properties (Numerical values)
Computer Vision Basics Image Terminology Binary Operations Filtering Edge Operators.
Biomedical Image Analysis and Machine Learning BMI 731 Winter 2005 Kun Huang Department of Biomedical Informatics Ohio State University.
Terms 1. VGA VGA - Short for Video Graphics Array, VGA is a popular display standard developed by IBM and introduced in 1987 VGA provides 640 x 480 resolution.
NA-MIC National Alliance for Medical Image Computing Interactive Editor tutorial Sonia Pujol, Ph.D. Surgical Planning Laboratory Harvard.
F. Beone, L. Capodieci, A. Mariano, M. Palmisano, E. Pesce, M. Re Motivations REMOTE LAB: an example with Helium Ion Microscope collaboration UTICT-UTTMATB.
 Process of partitioning an image into segments  Segments are called superpixels  Superpixels are made up several pixels that have similar properties.
L-Measure: a software tool for the analysis of neuronal morphology… …and its application to hippocampal pyramidal cells.

BRAIN ART How and Why We Study the Brain This slide-show was completed with the help of Roberto Gradini MD, PHD, Associate Professor of General Pathology,
OVERVIEW- What is GIS? A geographic information system (GIS) integrates hardware, software, and data for capturing, managing, analyzing, and displaying.
 In electrical engineering and computer science image processing is any form of signal processing for which the input is an image, such as a photograph.
Digital Image Processing & Analysis Spring Definitions Image Processing Image Analysis (Image Understanding) Computer Vision Low Level Processes:
Difference Between Raster and Vector Images Raster and vector are the two basic data structures for storing and manipulating images and graphics data on.
September 5, 2013Computer Vision Lecture 2: Digital Images 1 Computer Vision A simple two-stage model of computer vision: Image processing Scene analysis.
XP Practical PC, 3e Chapter 15 1 Creating Desktop Video and Animation.
Monitors and Sound Systems Section 3A. Objectives List the two most commonly used types of computer monitors. Explain how a CRT monitor displays images.
Intelligent Vision Systems Image Geometry and Acquisition ENT 496 Ms. HEMA C.R. Lecture 2.
Computer Graphics & Image Processing Lecture 1 Introduction.
Seeing © 2014 Project Lead The Way, Inc.Computer Science and Software Engineering.
NEURON CLASSIFICATION
Microscopes Compound Bright-Field Light Microscope
An Introduction to Analyzing Colors in a Digital Photograph Rob Snyder.
Lecture 7: Intro to Computer Graphics. Remember…… DIGITAL - Digital means discrete. DIGITAL - Digital means discrete. Digital representation is comprised.
Final project – Computational Biology RNA Quantification מגישים: מיכל סימון חיים בן שימול בהנחיית: יהודה ברודי ד"ר ירון שב-טל.
Graphics: Conceptual Model Real Object Human Eye Display Device Graphics System Synthetic Model Synthetic Camera Real Light Synthetic Light Source.
Microscopes. Micrographia Robert HookeRobert Hooke First report of cell structureFirst report of cell structure corkcork First illustrated book.
TechnoSpecialist Computers Information Package By Troy Rayner.
LCD Projectors and Document Cameras Defined Or everything you wanted to know but didn’t know who to ask...
COMPUTER GRAPHICS. Can refer to the number of pixels in a bitmapped image Can refer to the number of pixels in a bitmapped image The amount of space it.
Image-Pro Premier Basic Training Course Part 5 - Automated Counting.
Phase calibration pictures 3/9/10. Dark count image Required for: 1. determining image intensity in the absence of light. 2. also required for phase artifact.
Science 10 – Unit C BIOLOGY Chapter 1 – The Microscope.
Intelligent Vision Systems Image Geometry and Acquisition ENT 496 Ms. HEMA C.R. Lecture 2.
Parts of a Computer. Two Basic Components of a Computer System Hardware Parts of the Computer System you can physically touch Software Computer Instructions.
Quiz Week 8 Topical. Topical Quiz (Section 2) What is the difference between Computer Vision and Computer Graphics What is the difference between Computer.
Panel -Distinct segment of comic -Combination of images and text -Sectioned off by lines/borders called frames.
 Bright-field  Dark-field  Phase Contrast  Fluorescence.
Paresh Kamble Digital Image Processing Introduction by Paresh Kamble.
 Many people like the flexibility of digital images. For example:  They can be shared by attaching to /uploading to Internet  Sent via mobiles.
Date of download: 7/5/2016 Copyright © 2016 SPIE. All rights reserved. Spatial light modulator-two-photon microscope (SLM-2PM) scheme: (1) Ti:Sa laser.
Light Microscope.
Visual Information Processing. Human Perception V.S. Machine Perception  Human perception: pictorial information improvement for human interpretation.
Information Processes and Technology Information Processes Collecting.
DISPLAY DEVICES CIS 10, Group #1 April 01, 2006 C. X. A. L. K. H. A. V. ((( L. C.
Computer Imaging Systems
Detecting and Mixing Colors STEM DIGITAL Institute
Image Segmentation Classify pixels into groups having similar characteristics.
Real-time Computer Graphics Overview
Introduction to Computers
Deep brain single optical fiber fluorescence imaging
Volume 84, Issue 6, Pages (June 2003)
Volume 49, Issue 6, Pages (March 2006)
Neuroanatomy and Global Neuroscience
4 Characteristics of Light
LIGHT MICROSCOPY variations
Axons and Synaptic Boutons Are Highly Dynamic in Adult Visual Cortex
Volume 15, Issue 3, Pages (April 2016)
Synchrotron X-ray imaging provides micron resolution within a neocortical volume. a, Microscopic visualization of cells, blood vessels, and dendrites within.
Encoding Light Intensity by the Cone Photoreceptor Synapse
Presentation transcript:

Quantification des structures 3D à partir de coupes histologiques sériées R. Kraftsik.

X Programmes disponibles : l Neurolucida - PC l structures arborescentes, coupes sériée l Volume- SGI Indigo + G.T. l coupes sériées

Neurolucida Neuron Tracing: Trace and analyze branching neuron processes. Brain Mapping: Map, count cells, and measure their locations and morphology Morphometry: Make accurate anatomical maps and measurements of histologically distinct structures and regions

Neurolucida system: -Neurolucida Software -Image Overlay Device (Optical and/or Video based) -Microscope Stage Control Device -Tracing Device -Graphics Output Device -PC Computer System.

Neurolucida system, room color camera 640 X stepping stage + controller - Zeiss microscope, light and fluorescence Contact: R. Kraftsik

FEATURES: - Accurately overlays a computer graphics or text display onto the microscope image - High brightness intensity - Up to 1024x668 pixels resolution (PC and MAC) - Accessible controls for contrast and brightness - Does not interfere with conventional use of the microscope Lucivid

DRG - CR Sciatic nerve

Volume Program, SGI Contact: R. Kraftsik

Volume program, SGI. Contact R. Kraftsik Reconstruction of cortical neurons from EM sections

Volume program, SGI. Contact R. Kraftsik Reconstruction of dendritic segments from EM sections

Feedback: Privacy Policy Feedback
Do Not Sell My Personal Information
About project: SlidePlayer Terms of Service

© 2025 SlidePlayer.com. Inc. All rights reserved.