Mobilization of copper ions from insoluble copper compounds by methanobactin Nay Myo Win, Joe Heimann, Shane Hodgson & Dr. Scott Hartsel  Chemistry Department.

Slides:



Advertisements
Similar presentations
Silver Alloy lab. Warm-up Mg + O 2 MgO What is the % composition of Mg in MgO? If I start with a 5.0g sample of Mg, what mass of MgO should I get (assuming.
Advertisements

The characteristics of water that make it important to living things.
Soil Fertility and Nutrient Bioavailability Sponsored by the DEST program China Higher Education Strategic Initiatives © The University of Adelaide.
254nm 282nm 340nm 302nm ELIMINATING INTERNAL REDUCTANTS A.Methionine: susceptible to oxidation. However, this amino acid is not present in all samples.
Our consultation services and technologies reflect a wide range of technical expertise.
Chemistry C Atomic Structure
Chapter 4 Biogeochemical Cycles. Objectives:  Identify and describe the flow of nutrients in each biogeochemical cycle.  Explain the impact that humans.
Oxidation and Reduction. Overview Oxidation and reduction reactions always occur together (redox reactions) You can’t have one without the other Includes:
E NVIRONMENTAL CHEMISTRY E 12. water and soil. W ATER AND SOIL Solve problems relating to the removal of heavy- metal ions, phosphates and nitrates from.
The Alkali Metals (Group 1 Except Hydrogen)
An Investigation of the Interactions Between Zinc-deficient and Copper, Zinc Superoxide Dismutase Katie Meyers Dr. Joe Beckman Department of Biochemistry/Biophysics.
Chemical Basis for Formation of Ores How do we get a lot of one element in a small place? Ore: a body of rock with a relatively high concentration of an.
TRACE METALS - FROM DEFICIENCY TO TOXICITY Quest – July 22, 2004 Yeala Shaked, Yan Xu and Francois Morel, Geosciences Dept, Ecology and Evolutionary Biology.
Assessing Aquatic Ecosystems & Measurement. Aquatic Ecosystem Assessment The health of an aquatic ecosystem can be determined by examining a variety of.
Copper Binding of Mutant Quad SOD1
Soil Washing “There is a need for increased use of new separation technologies (such as soil washing) that reduce the quantity of waste requiring solidification/stabilization,
An Introduction to Acid Mine Drainage by George Mitchell and Tim Craddock.
Chapter 16: Aqueous Ionic Equilibria Common Ion Effect Buffer Solutions Titrations Solubility Precipitation Complex Ion Equilibria.
Nutrient Balance NRES 406/606 Spring 2013
Lesson 25 Percent Composition
Chemical Weathering. I. Introduction Chemical Weathering I. Introduction II. Process of Decomposition A. Overview: Decomposition alters minerals into.
BIOCYANIDE DEMONSTRATION PROJECT Activity III ; Project 5.
Determination of Metals
1. 2 Vitamin Losses During Food Storage and Processing 16-June-2008.
Hydrometallurgy Conference Indigenous microorganism strains as bio- extractants of Ca, Fe and Mg from metallurgical and mine drainages By E. Fosso.
Chemical compounds lesson-2 unit-1 Made by: Dr. Niveen Fawzy.
Water Chemistry: pH. pH pH is the measure of hydrogen ions (H+) –Negative logarithm of the H+ concentration Higher the pH, the lower the H+ concentration.
Aquatic Ecosystem Chemistry Dissolved Oxygen (DO) Temperature Redox Potential (ORP) Major ions (Hardness; Conductivity; Salinity/TDS) Carbonate Buffer.
Abstract/Background Worldwide, corrosion of drinking water pipes and build-up of scales on the interior pipe wall impacts both the quality and quantity.
Shipwrecks, Corrosion and Conservation
Stoichiometry Stoicheion from Greek which means first principle or element and metron is a measure Stoichiometry is the quantitative relationship among.
MINERAL FAMILIES.
The Chemical Level of Organization Chapter 2. Chemistry Review  Matter – anything that occupies space and has weight  Atom – smallest stable unit of.
Characterization of 3-OHFT 3-hydroxyflavothione was characterized by 1 H NMR, UV-Visible spectroscopy, LCMS and HPLC. This synthetic approach has resulted.
MODULE C5: CHEMICALS OF THE NATURAL ENVIRONMENT. Atmosphere Molecular elements (e.g. oxygen, nitrogen, ozone) and compounds (e.g.carbon dioxide, water)
Bellringer.
(E6) Water Treatment Sarah Black.
One of the most common serious diseases of the developing world is almost unknown in this country. It is Leishmanaisis, also known as kala- azar. After.
MATTER CYCLING IN ECOSYSTEMS Nutrient Cycles: Global Recycling –Global Cycles recycle nutrients through the earth’s air, land, water, and living organisms.
Chapter 3 Ecosystems: What Are They and How Do They Work?
PART -III Analytical Methods for Metal Speciation in Water and Solids
Chapter 5 Section 2 The Cycling of Materials. Objectives List the three stages of the carbon cycle. Describe where fossil fuels are located. Identify.
1 2 It Matters! 3 Energy 4 Chemical Bonds 5 Oxidation - Reduction.
Fig. S2. In vitro characterization of ligand binding properties of recombinant ThiY and RibY from C. aurantiacus. (A) Identification of ThiY-bound ligand.
“ Safer, More Effective ISCO Remedial Actions Using Non-Extreme Persulfate Activation to Yield Sustained Secondary Treatment ” Michael Scalzi, President.
Chapter 4 Biogeochemical Cycles. Objectives:  Identify and describe the flow of nutrients in each biogeochemical cycle.  Explain the impact that humans.
Chapter 3 Biogeochemical Cycles. Objectives:  Identify and describe the flow of nutrients in each biogeochemical cycle.  Explain the impact that humans.
Total Bacterial Protein Isolation. A bacterial protein is a protein which is either part of the structure of the bacterium OR produced by bacterium as.
Abstract Synthesis Method Ultraviolet-visible Spectroscopy (UV-Vis) Methanobactin is a biological molecule that is secreted by methanotrophic bacteria.
Hydrogen.
Total Bacterial Protein Isolation
Presentation of Redox By: Seth Princinsky, Joe bale, Brianna scott.
Bacterial Growth. I. Determine in terms of population size. Nature there is a mixture of organisms living together. Nature there is a mixture of organisms.
Reactions of Metals. Metal Oxides Salts and hydrogen React with oxygen to giveReact with acids to give.
Extracting metals.
Drinking Water & Dissolved Oxygen in Water. Water Highly polar Forms Hydrogen bonds Has the ability to dissolve many chemicals.
Drug detection and analysis
Compost pH. pH pH decreases as the [H+] increases pH range is 0-14 At 25C the pH of an acidic solution is less than 7.00 At 25C the pH of a basic solution.
Experiments in Analytical Chemistry -EDTA determination of Ca and Mg in water.
Olivia F. Hurst, Dana Warwick, Dr. Roslyn Theisen , and Dr
Bioremediation of heavy- and radioactive-metal contaminations from soil and ground water John Hanna.
CHEMICAL WEATHERING.
Chemical Weathering.
The Earth’s Resources 1. Sustainable Development
Chemical Weathering SAPROLITE.
Course Instructor: Muktadir S. Hossain (MSH3) FALL
Metabolism and Survival
Evaluating claims that human activities are not causing climate change
C4 – Chemical changes Key Concepts.
BY HALAVATH RAMESH 16-MCH-001 DEPARTMENT OF CHEMISTRY LOYOLA COLLEGE –CHENNAI UNIVERSITY OF MADRAS Protein Purification.
Presentation transcript:

Mobilization of copper ions from insoluble copper compounds by methanobactin Nay Myo Win, Joe Heimann, Shane Hodgson & Dr. Scott Hartsel  Chemistry Department  University of Wisconsin-Eau Claire Abstract Background Results MethodConclusion References  Copper is an essential nutrient for life as well as environmental toxin. Microbes are likely a very important part of the global copper cycle and the bioweathering of copper minerals. We have looked at the ability of a secreted bacterial copper-binding molecule, methanobactin, to mobilize copper from pure insoluble copper oxides and sulfides as models for bioweathering of corresponding natural copper minerals.  We followed the mobilization of copper spectrophotometrically in the presence of methanobactin and other known chemical copper binding agents. We find that methanobactin has a very high affinity for both Copper (I) and Copper(II) and can easily extract copper from all of these minerals, in particular Cu(I) minerals (saturation reached in less than 2 hours for Cu(I) minerals).  In addition unlike another Cu(I) chelating compound, BCS, methanobactin can stabilize the normally unstable Cu(I) ion for long periods of time even in the presence of water and oxygen.  These data suggest that in areas where these methanotrophic bacteria are common, secreted methanobactin could have a significant impact on soluble copper concentrations and weathering of copper- containing minerals.. Acknowledgements  University of Wisconsin – Eau Claire Chemistry Department  University of Wisconsin – Eau Claire Office of Research and Sponsored Programs (ORSP)  University of Wisconsin – Eau Claire Learning and Technology Services  What is methanobactin?  A polypeptide produced by the methanotrophs to collect copper from their surroundings  Can bind to other variety of earth metals with varying affinities  Why is it important?  Copper is necessary for methanotropic (methane- eating) bacteria to degrade methane and other organic molecules  Excess copper in the environment is toxic to living cells  Weathering processes  Oil spills cleaned up by methanotrophs  Extraction of copper from ores  Spectrophotometry The data were collected by using spectrophotometer. Different copper compounds, Copper (I) oxide, Copper (II) Oxide, Copper (I) sulfide, and Copper (II) sulfide, were used to determine the copper binding affinity of Bathocuproinedisulfonic acid (BCS), which is a known copper chelating agent, and methanobactin.  BCS - Copper bound BCS shows peak at 480 nm. - Spectra were taken 30 min/ cycle for 24 hours period.  Methanobactin - Copper bound mb loses absorbance at 394 nm. - Spectra were taken 15 min/ cycle for hours period.  ICP-MS (Inductvely coupled plasma mass spectrometry) Copper compounds were incubated in MOPS buffer overnight and tested for availability of copper ions in water.  Methanobactin can rapidly remove copper ions from copper minerals and compounds normally considered to be completely insoluble in water.  The abundance of methanotrophs in nature suggests that MB could have a significant impact on mobilizing and weathering of copper minerals in the environment.  MB is at least as effective as the high-affinity specific Copper(I) chelator BCS at mobilizing copper and is more stable over time. Copper (I) Oxide Copper (II) Oxide Copper (I) Sulfide Copper (II) Sulfide Results from ICP-MS showing the insolubility of copper compounds in MOPS buffer Digenite (Cu9S5) with MB for 48 hours 1.Chi Fru, E. (2011). Copper Biogeochemistry: A Cornerstone in Aerobic Methanotrophic Bacterial Ecology and Activity. Geomicrobiology Journal, 28: Campos, C., et. Al (2009). Evaluation of the copper (II) reduction assay using bathocuproinedisulfonic acid disodium salt for the total antioxidant capacity assessment: The CUPRAC-BCS assay. Analytical Biochemisty 392 (2009) Concentration (M)Standard Deviation (M) MOPS2.28E E-09 CuO3.30E E-09 Cu2O6.73E E-08 CuS9.87E E-08 Cu2S1.39E E-08