1,000,000 1,000,000,000 PCR cycles 20 40 30 Theorectical yield Actual yield “Plateau effect” No. of DNA copies How much DNA amplification has occurred?

Slides:

Advertisements

Similar presentations

Northern blotting. Electrophoresis We can separate DNA and RNA molecules by size using agarose gel electrophoresis.

Advertisements

Biotechniques. Gel electrophoresis Separates molecules according to size and charge. Different sized segments of DNA cut by restriction enzymes Segments.

Gel Electrophoresis.

Agarose gel electrophoresis

Gel Electrophoresis of DNA Molecular Genetics Presentation by: Nana Sugma Mulyana Febrina Anggraini Ginting Faizal Dony Rifai Nor Aviva.

Gel electrophoresis Ashti Mohammad Amin M.Sc. Molecular Biology Medical Research Center Hawler Medical University

BRIDGES 2014 Agarose Gel Visualization of Restriction Enzyme Digest.

Biotech Lab #5 DNA Goes to the Races “Gel electrophoresis”

Outbreak Lab: In this lab, biotech procedures will be used to see if a sample of viral DNA is the deadly Alabama virus. The specific technique that you.

SEPARATION OF DNA FRAGMENTS BY LENGTH. Organic molecules such as DNA are charged. DNA is negatively charged because the phosphates (red circles) that.

“Gel electrophoresis”. Gel electrophoresis is a procedure for separating a mixture of molecules through a stationary material (gel) in an electrical field.

ENDURO Horizontal Electrophoresis Systems. Casting a Gel Attach a buffer dam to one side of gel tray Attach the other buffer dam to the other side of.

General Genetics.  To learn how to prepare agarose gel electrophoresis.

Lab. 3 Gel Electrophoresis

Gel electrophoresis equipment. Gel electrophoresis One of the most common experiments in life science field. Gel electrophoresis itself isn’t complete.

Prepered by:- Rana Al-Turki

DNA-Fingerprint1 Detection of PCR Products by Agarose Gel Electrophoresis.

Agarose Gel Electrophoresis

Agarose gel electrophoresis

Collect Buccal Cells. PCR Polymerase Chain Reaction DNA/gene amplification.

4.4 Using Gel Electrophoresis to Study Gene Molecules

Part 1. Gel electrophoresis DNA is negatively charged (because of phosphate backbone) DNA will be attracted to positively charged poles and repelled from.

Electrophoresis Electrophoresis is the movement of molecules by an electric current .This is practically done in a matrix to limit migration and contain.

Gel Electrophoresis based on motion of charged molecules in an electric field toward the opposite charge. Agarose gels (for larger fragments of DNA) or.

Recombinant DNA Technology

Lab.8 8RBs0Ghg_48

Agarose (Horizontal) Gel Electrophoresis Malasian word for seaweed is “agar-agar”. Agarose is derived from red seaweed. Electrophoresis means “carrying.

 DNA (gene mutations, paternity, organs compatibility for transplantations)  RNA  Proteins (gene expression)

PCR is DNA replication in a test tube Ex. 25: PCR Based Testing for Water Contaminants Day 2.

Introduction to Gel Electrophoresis. Outline How to prepare a gel How to micropipet Practice setting up electrophoresis Discussion viewing of gel –In.

GEL ELECTROPHORESIS. FIRST, WHAT……  Simply put, gel electrophoresis is a technique used to separate molecules such a DNA, RNA and proteins according.

Gel Electrophoresis.

(RFLP Electrophoresis)

Gel Electrophoresis. Definition – COPY ME! Separation of DNA fragments according to size and charge Based on movement through a gel medium when an.

Polymerase Chain Reaction (DNA Polymerase – duplicates DNA when cells divide) DNA copying machine – creates the compliment strand (ATCG-TAGC)

Agarose gel electrophoresis. Agarose gel electrophoresis is an easy way to separate DNA fragments by their sizes and visualize them. It is a common diagnostic.

Gel Electrophoresis.  This workforce solution was funded by a grant awarded under the President’s High Growth Job Training Initiative as implemented.

Gel Electrophoresis of DNA. DNA as Forensic Evidence Individual evidence – identify a single person Trace evidence – small amount left at crime scene.

Gel Electrophoresis.

C Biochemistry clinical practice CLS 432

Molecular Genetic Technologies Gel Electrophoresis PCR Restriction & ligation Enzymes Recombinant plasmids and transformation DNA microarrays DNA profiling.

Gel Electrophoresis of DNA. What is Gel Electrophoresis? Electro = flow of electricity, phoresis, from the Greek = to carry across A gel is a colloid,

Page Gel Electrophoresis gel electrophoresis – moving DNA through a gel medium using an electric current Why can we move DNA with electricity?

Gel Electrophoresis What is Gel Electrophoresis? Gel electrophoresis separates molecules on the basis of their charge and size. The charged macromolecules.

Lab.3 Gel electrophoresis

Estimation of Nucleic Acid NAHLA BAKHAMIS. 1.Agarose Gel Electrophoresis: Separation and analysing DNA of varying sizes, by moving –ve charge na through.

DO NOW 1. Get out your Embryos-R-Us HW 2. Detail the decision you made. Why did you make that decision?

Part Power DNA How fast will the DNA migrate? strength of the electrical field, buffer, density of agarose gel… Size of the DNA! *Small DNA move.

PCR Polymerase chain reaction. PCR is a method of amplifying (=copy) a target sequence of DNA.

Gel electrophoresis.

Agarose Gel Electrophoresis

List general characteristics of all races

PCR - Electrophoresis Adding primers to the DNA for the PCR process.

Practical Of Genetics Gel electrophoresis.

Outbreak Lab: In this lab, biotech procedures will be used to see if a sample of viral DNA is the deadly Alabama virus. The specific technique that you.

Lab#.3 Gel electrophoresis

Gel Electrophoresis of DNA

Gel Electrophoresis Method of separating molecules within an electric field based on the size and charge of DNA fragments.

Introduction to Gel Electrophoresis

Biotech Lab #3 DNA Goes to the Races

Introduction to Gel Electrophoresis

Agarose gel Electrophoresis

Introduction to Gel Electrophoresis

Electrophoresis… an analysis tool.

Creating a DNA Fingerprint by Gel Electrophoresis

Gel Electrophoresis Ms. Cuthrell.

Separating molecules by size and charge

Another way to separate mixtures!

ELECTROPHORESIS of serum proteins and dna

Presentation transcript:

1,000,000 1,000,000,000 PCR cycles Theorectical yield Actual yield “Plateau effect” No. of DNA copies How much DNA amplification has occurred?

How do scientists analyse these PCR products?

Agarose Gel Electrophoresis Separates DNA fragments according to their size

Step 1 – Agarose is added to buffer and heated to 95ºC (to melt it). After cooling, it is poured into a pre-prepared casting tray Step 2 – a comb is inserted immediately to create wells for loading the samples Step 3 – the gel is left to set Step 4 – the comb is removed slowly (see set of wells that have been created) Making Agarose Gel

Agarose gel Well 1 Well 2 Well 3 Well 4 Well 5 Making Agarose Gel A fluorophore (DNA SafeView) is added to the gel. This intercalates with the DNA & fluoresces when excited by UV light

Agarose gel Loading DNA samples

Agarose gel Loading DNA samples

Separating the DNA Fragments After the samples are loaded into the wells, an electric current is applied across the gel and the gel is “run”

__++ Separating the DNA Fragments

__++

__++

Visualising DNA The UV light excites the DNA SafeView (fluorophore) that is bound to the DNA